The number of nephrons in different glomerular diseases.

Blood pressure Diabetic nephropathy IgA nephropathy Membranous nephropathy Minimal change disease Nephritic syndrome Nephron number Nephrotic syndrome Podocytes eGFR

Journal

PeerJ
ISSN: 2167-8359
Titre abrégé: PeerJ
Pays: United States
ID NLM: 101603425

Informations de publication

Date de publication:
2019
Historique:
received: 01 04 2019
accepted: 07 08 2019
entrez: 20 9 2019
pubmed: 20 9 2019
medline: 20 9 2019
Statut: epublish

Résumé

The total number of nephrons has been measured mainly from post-mortem studies and only in selected populations. Data from living subjects are scanty, and direct comparisons among different glomerular diseases are lacking. The present work exploits modern methodology to estimate the total nephron number in glomerulopathies with prevalent proteinuria/nephrotic syndrome versus glomerulopathies with nephritic syndrome (IgA nephropathy (IgAN), lupus nephritis), thus extending previous observations about the number and function of glomeruli in different physiological and pathological states. This is a retrospective study based on one hundred and seven patients who have undergone renal biopsy. The glomerular density has been estimated from the biopsy specimens and the total cortical volume has been obtained from ultrasound recordings. Stereological methods have been applied to calculate the total number of nephrons and their volume. The correlation between clinical parameters and quantitative morphological data have studied using the Pearson correlation coefficient ( The total number of nephrons inversely correlated with the systolic blood pressure ( The fusion of the podocyte foot-processes that typically occurs in purely proteinuric diseases does not modify the glomerular filtration rate: therefore in these situations, the change in eGFR depends mainly on the number of available glomeruli. On the other side, the eGFR decrease occurring in nephritic syndromes, such as IgAN, cannot be explained simply on the basis of the number of NSG and likely depends on the substantial involvement of the mesangial axis. Future studies should verify whether these changes are reversible with appropriate therapy, thus reversing eGFR decrease.

Sections du résumé

BACKGROUND BACKGROUND
The total number of nephrons has been measured mainly from post-mortem studies and only in selected populations. Data from living subjects are scanty, and direct comparisons among different glomerular diseases are lacking. The present work exploits modern methodology to estimate the total nephron number in glomerulopathies with prevalent proteinuria/nephrotic syndrome versus glomerulopathies with nephritic syndrome (IgA nephropathy (IgAN), lupus nephritis), thus extending previous observations about the number and function of glomeruli in different physiological and pathological states.
METHODS METHODS
This is a retrospective study based on one hundred and seven patients who have undergone renal biopsy. The glomerular density has been estimated from the biopsy specimens and the total cortical volume has been obtained from ultrasound recordings. Stereological methods have been applied to calculate the total number of nephrons and their volume. The correlation between clinical parameters and quantitative morphological data have studied using the Pearson correlation coefficient (
RESULTS RESULTS
The total number of nephrons inversely correlated with the systolic blood pressure (
DISCUSSION CONCLUSIONS
The fusion of the podocyte foot-processes that typically occurs in purely proteinuric diseases does not modify the glomerular filtration rate: therefore in these situations, the change in eGFR depends mainly on the number of available glomeruli. On the other side, the eGFR decrease occurring in nephritic syndromes, such as IgAN, cannot be explained simply on the basis of the number of NSG and likely depends on the substantial involvement of the mesangial axis. Future studies should verify whether these changes are reversible with appropriate therapy, thus reversing eGFR decrease.

Identifiants

pubmed: 31534861
doi: 10.7717/peerj.7640
pii: 7640
pmc: PMC6731770
doi:

Types de publication

Journal Article

Langues

eng

Pagination

e7640

Déclaration de conflit d'intérêts

The authors declare that they have no competing interests.

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Auteurs

Davide Viggiano (D)

Department of Medicine and Health Sciences, University of Molise, Campobasso, Italy.

Michelangelo Nigro (M)

UOC of Nephrology and dialysis, Eboli Hospital "Maria SS Addolorata", Eboli, Italy.

Francesco Sessa (F)

Department of Urologic Robotic Surgery and Renal Transplantation, University of Florence, Careggi Hospital, Florence, Italy.
Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy.

Graziano Vignolini (G)

Department of Urologic Robotic Surgery and Renal Transplantation, University of Florence, Careggi Hospital, Florence, Italy.
Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy.

Riccardo Campi (R)

Department of Urologic Robotic Surgery and Renal Transplantation, University of Florence, Careggi Hospital, Florence, Italy.
Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy.

Sergio Serni (S)

Department of Urologic Robotic Surgery and Renal Transplantation, University of Florence, Careggi Hospital, Florence, Italy.
Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy.

Rosa Maria Pollastro (RM)

Department of Translational Medicine, University of Campania "L. Vanvitelli", Naples, Italy.

Gianfranco Vallone (G)

Department of Radiology, University of Naples "Federico II", Naples, Italy.

Giuseppe Gigliotti (G)

UOC of Nephrology and dialysis, Eboli Hospital "Maria SS Addolorata", Eboli, Italy.

Giovambattista Capasso (G)

Department of Translational Medicine, University of Campania "L. Vanvitelli", Naples, Italy.
Biogem, Ariano Irpino, Italy.

Classifications MeSH