TRPC channels regulate Ca2+-signaling and short-term plasticity of fast glutamatergic synapses.
Journal
PLoS biology
ISSN: 1545-7885
Titre abrégé: PLoS Biol
Pays: United States
ID NLM: 101183755
Informations de publication
Date de publication:
09 2019
09 2019
Historique:
received:
08
03
2019
accepted:
29
08
2019
revised:
01
10
2019
pubmed:
20
9
2019
medline:
6
2
2020
entrez:
20
9
2019
Statut:
epublish
Résumé
Transient receptor potential (TRP) proteins form Ca2+-permeable, nonselective cation channels, but their role in neuronal Ca2+ homeostasis is elusive. In the present paper, we show that TRPC channels potently regulate synaptic plasticity by changing the presynaptic Ca2+-homeostasis of hippocampal neurons. Specifically, loss of TRPC1/C4/C5 channels decreases basal-evoked secretion, reduces the pool size of readily releasable vesicles, and accelerates synaptic depression during high-frequency stimulation (HFS). In contrast, primary TRPC5 channel-expressing neurons, identified by a novel TRPC5-τ-green fluorescent protein (τGFP) knockin mouse line, show strong short-term enhancement (STE) of synaptic signaling during HFS, indicating a key role of TRPC5 in short-term plasticity. Lentiviral expression of either TRPC1 or TRPC5 turns classic synaptic depression of wild-type neurons into STE, demonstrating that TRPCs are instrumental in regulating synaptic plasticity. Presynaptic Ca2+ imaging shows that TRPC activity strongly boosts synaptic Ca2+ dynamics, showing that TRPC channels provide an additional presynaptic Ca2+ entry pathway, which efficiently regulates synaptic strength and plasticity.
Identifiants
pubmed: 31536487
doi: 10.1371/journal.pbio.3000445
pii: PBIOLOGY-D-19-00663
pmc: PMC6773422
doi:
Substances chimiques
Calcium Channels
0
TRPC Cation Channels
0
Glutamine
0RH81L854J
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e3000445Déclaration de conflit d'intérêts
The authors declare no competing financial interest.
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