The ERC1 scaffold protein implicated in cell motility drives the assembly of a liquid phase.
Adaptor Proteins, Signal Transducing
/ metabolism
Animals
COS Cells
Cell Line, Tumor
Cell Membrane
/ metabolism
Cell Movement
/ physiology
Chlorocebus aethiops
Cytoplasm
/ metabolism
Humans
Nerve Tissue Proteins
/ metabolism
Nuclear Matrix-Associated Proteins
/ metabolism
rab GTP-Binding Proteins
/ metabolism
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
19 09 2019
19 09 2019
Historique:
received:
13
03
2019
accepted:
07
08
2019
entrez:
21
9
2019
pubmed:
21
9
2019
medline:
28
10
2020
Statut:
epublish
Résumé
Several cellular processes depend on networks of proteins assembled at specific sites near the plasma membrane. Scaffold proteins assemble these networks by recruiting relevant molecules. The scaffold protein ERC1/ELKS and its partners promote cell migration and invasion, and assemble into dynamic networks at the protruding edge of cells. Here by electron microscopy and single molecule analysis we identify ERC1 as an extended flexible dimer. We found that ERC1 scaffolds form cytoplasmic condensates with a behavior that is consistent with liquid phases that are modulated by a predicted disordered region of ERC1. These condensates specifically host partners of a network relevant to cell motility, including liprin-α1, which was unnecessary for the formation of condensates, but influenced their dynamic behavior. Phase separation at specific sites of the cell periphery may represent an elegant mechanism to control the assembly and turnover of dynamic scaffolds needed for the spatial localization and processing of molecules.
Identifiants
pubmed: 31537859
doi: 10.1038/s41598-019-49630-y
pii: 10.1038/s41598-019-49630-y
pmc: PMC6753080
doi:
Substances chimiques
Adaptor Proteins, Signal Transducing
0
Erc1 protein, mouse
0
Nerve Tissue Proteins
0
Nuclear Matrix-Associated Proteins
0
rab GTP-Binding Proteins
EC 3.6.5.2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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