Genomic diversity, linkage disequilibrium and selection signatures in European local pig breeds assessed with a high density SNP chip.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
19 09 2019
Historique:
received: 06 03 2019
accepted: 30 08 2019
entrez: 21 9 2019
pubmed: 21 9 2019
medline: 28 10 2020
Statut: epublish

Résumé

Genetic characterization of local breeds is essential to preserve their genomic variability, to advance conservation policies and to contribute to their promotion and sustainability. Genomic diversity of twenty European local pig breeds and a small sample of Spanish wild pigs was assessed using high density SNP chips. A total of 992 DNA samples were analyzed with the GeneSeek Genomic Profiler (GGP) 70 K HD porcine genotyping chip. Genotype data was employed to compute genetic diversity, population differentiation and structure, genetic distances, linkage disequilibrium and effective population size. Our results point out several breeds, such as Turopolje, Apulo Calabrese, Casertana, Mora Romagnola and Lithuanian indigenous wattle, having the lowest genetic diversity, supported by low heterozygosity and very small effective population size, demonstrating the need of enhanced conservation strategies. Principal components analysis showed the clustering of the individuals of the same breed, with few breeds being clearly isolated from the rest. Several breeds were partially overlapped, suggesting genetic closeness, which was particularly marked in the case of Iberian and Alentejana breeds. Spanish wild boar was also narrowly related to other western populations, in agreement with recurrent admixture between wild and domestic animals. We also searched across the genome for loci under diversifying selection based on F

Identifiants

pubmed: 31537860
doi: 10.1038/s41598-019-49830-6
pii: 10.1038/s41598-019-49830-6
pmc: PMC6753209
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

13546

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Auteurs

M Muñoz (M)

Departamento Mejora Genética Animal, INIA, Madrid, Spain.

R Bozzi (R)

DAGRI, Animal Science Section, Università degli Studi di Firenze, Firenze, Italy.

J García-Casco (J)

Departamento Mejora Genética Animal, INIA, Madrid, Spain.

Y Núñez (Y)

Departamento Mejora Genética Animal, INIA, Madrid, Spain.

A Ribani (A)

Department of Agricultural and Food Sciences, University of Bologna, Bologna, Italy.

O Franci (O)

DAGRI, Animal Science Section, Università degli Studi di Firenze, Firenze, Italy.

F García (F)

Departamento Mejora Genética Animal, INIA, Madrid, Spain.

M Škrlep (M)

Kmetijski inštitut Slovenije, Hacquetova ulica 17, SI-1000, Ljubljana, Slovenia.

G Schiavo (G)

Department of Agricultural and Food Sciences, University of Bologna, Bologna, Italy.

S Bovo (S)

Department of Agricultural and Food Sciences, University of Bologna, Bologna, Italy.

V J Utzeri (VJ)

Department of Agricultural and Food Sciences, University of Bologna, Bologna, Italy.

R Charneca (R)

Instituto de Ciências Agrárias e Ambientais Mediterrânicas (ICAAM), Universidade de Évora, Évora, Portugal.

J M Martins (JM)

Instituto de Ciências Agrárias e Ambientais Mediterrânicas (ICAAM), Universidade de Évora, Évora, Portugal.

R Quintanilla (R)

IRTA, Programa de Genética y Mejora Animal, Barcelona, Spain.

J Tibau (J)

IRTA, Programa de Genética y Mejora Animal, Barcelona, Spain.

V Margeta (V)

Faculty of Agrobiotechnical Sciences Osijek, University of Osijek, Osijek, Croatia.

I Djurkin-Kušec (I)

Faculty of Agrobiotechnical Sciences Osijek, University of Osijek, Osijek, Croatia.

M J Mercat (MJ)

IFIP - Institut du Porc, Le Rheu, France.

J Riquet (J)

INRA, Génétique Physiologie et Système d'Elevage, Castanet-Tolosan, France.

J Estellé (J)

GABI, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France.

C Zimmer (C)

Bäuerliche Erzeugergemeinschaft Schwäbisch Hall, Wolpertshausen, Germany.

V Razmaite (V)

Animal Science Institute, Lithuanian University of Health Sciences, Baisogala, Lithuania.

J P Araujo (JP)

Centro de Investigação de Montanha (CIMO), Instituto Politécnico de Viana do Castelo, Escola Superior Agrária, Ponte de Lima, Portugal.

Č Radović (Č)

Institute for Animal Husbandry-Pig Research Department, Autoput for Zagreb 16, 11080, Belgrade-Zemun, Serbia.

R Savić (R)

University of Belgrade, Faculty of agriculture, Nemanjina 6, 11080, Belgrade-Zemun, Serbia.

D Karolyi (D)

Department of Animal Science, University of Zagreb, Faculty of Agriculture, Zagreb, Croatia.

M Gallo (M)

Associazione Nazionale Allevatori Suini (ANAS), Roma, Italy.

M Čandek-Potokar (M)

Kmetijski inštitut Slovenije, Hacquetova ulica 17, SI-1000, Ljubljana, Slovenia.

A I Fernández (AI)

Departamento Mejora Genética Animal, INIA, Madrid, Spain.

L Fontanesi (L)

Department of Agricultural and Food Sciences, University of Bologna, Bologna, Italy.

C Óvilo (C)

Departamento Mejora Genética Animal, INIA, Madrid, Spain. ovilo@inia.es.

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Classifications MeSH