Multivariate Analysis of Difference Raman Spectra of the Irradiated Nucleus and Cytoplasm Region of SH-SY5Y Human Neuroblastoma Cells.
Principal Component Analysis
Raman micro-spectroscopy
effects of X-rays on cell nucleus and cytoplasm
single SH-SY5Y human cancer cells
Journal
Sensors (Basel, Switzerland)
ISSN: 1424-8220
Titre abrégé: Sensors (Basel)
Pays: Switzerland
ID NLM: 101204366
Informations de publication
Date de publication:
14 Sep 2019
14 Sep 2019
Historique:
received:
26
08
2019
revised:
10
09
2019
accepted:
13
09
2019
entrez:
22
9
2019
pubmed:
22
9
2019
medline:
7
2
2020
Statut:
epublish
Résumé
Previous works showed that spatially resolved Raman spectra of cytoplasm and nucleus region of single cells exposed to X-rays evidence different features. The present work aims to introduce a new approach to profit from these differences to deeper investigate X-ray irradiation effects on single SH-SY5Y human neuroblastoma cells. For this aim, Raman micro-spectroscopy was performed in vitro on single cells after irradiation by graded X-ray doses (2, 4, 6, 8 Gy). Spectra from nucleus and cytoplasm regions were selectively acquired. The examination by interval Principal Component Analysis (i-PCA) of the difference spectra obtained by subtracting each cytoplasm-related spectrum from the corresponding one detected at the nucleus enabled us to reveal the subtle modifications of Raman features specific of different spatial cell regions. They were discussed in terms of effects induced by X-ray irradiation on DNA/RNA, lipids, and proteins. The proposed approach enabled us to evidence some features not outlined in previous investigations.
Identifiants
pubmed: 31540064
pii: s19183971
doi: 10.3390/s19183971
pmc: PMC6766837
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Références
Faraday Discuss. 2016 Jun 23;187:213-34
pubmed: 27043923
Appl Spectrosc. 2015 Jul;69(7):834-42
pubmed: 26037638
Nat Protoc. 2016 Apr;11(4):664-87
pubmed: 26963630
J Biomed Opt. 2018 Jun;23(7):1-23
pubmed: 29956506
Int J Radiat Biol. 2019 Feb;95(2):207-214
pubmed: 30496011
Phys Med Biol. 2000 Feb;45(2):R1-59
pubmed: 10701500
Opt Express. 2012 Sep 24;20(20):22158-71
pubmed: 23037364
J Biomed Opt. 2017 Nov;22(11):1-10
pubmed: 29110445
NPJ Regen Med. 2017 May 15;2:12
pubmed: 29302348
Chem Soc Rev. 2016 Apr 7;45(7):1879-900
pubmed: 26497386
Front Mol Biosci. 2014 Nov 17;1:24
pubmed: 25988165
Analyst. 2018 Aug 6;143(16):3850-3858
pubmed: 30004539
Appl Spectrosc. 2015;69(2):193-204
pubmed: 25588147
J Biomed Opt. 2015 Mar;20(3):035003
pubmed: 25769498
RSC Adv. 2018 Jul 20;8(46):25888-25908
pubmed: 35541973
J Biophotonics. 2009 Feb;2(1-2):47-69
pubmed: 19343685
Sci Rep. 2016 Feb 17;6:21006
pubmed: 26883914
Chemometr Intell Lab Syst. 2011 Dec 15;109(2):162-170
pubmed: 26246647
Appl Opt. 2018 Aug 1;57(22):E11-E19
pubmed: 30117916
Cancer Detect Prev. 2006;30(6):515-22
pubmed: 17113723
Appl Spectrosc. 2003 Nov;57(11):1363-7
pubmed: 14658149
Adv Drug Deliv Rev. 2015 Jul 15;89:71-90
pubmed: 25728764
Radiat Res. 2015 Apr;183(4):407-16
pubmed: 25844945
Appl Spectrosc. 2011 Sep;65(9):1009-16
pubmed: 21929855
Analyst. 2012 Jan 21;137(2):322-32
pubmed: 22114757
Anal Bioanal Chem. 2010 Mar;396(5):1781-91
pubmed: 20087730
Radiat Res. 2018 May;189(5):497-504
pubmed: 29474157
Exp Mol Med. 2007 Oct 31;39(5):583-93
pubmed: 18059134
Int J Radiat Biol. 2019 Jan;95(1):44-53
pubmed: 29528761
Analyst. 2019 Mar 11;144(6):2097-2108
pubmed: 30735207
Sensors (Basel). 2018 Jul 12;18(7):
pubmed: 30002282
J Pharm Biomed Anal. 2019 Feb 5;164:557-573
pubmed: 30466024
Phys Med Biol. 2011 Jan 7;56(1):19-38
pubmed: 21119222