Single-Cell Analysis of CD4 T Cells in Type 1 Diabetes: From Mouse to Man, How to Perform Mechanistic Studies.
Journal
Diabetes
ISSN: 1939-327X
Titre abrégé: Diabetes
Pays: United States
ID NLM: 0372763
Informations de publication
Date de publication:
10 2019
10 2019
Historique:
received:
16
07
2019
accepted:
21
07
2019
entrez:
22
9
2019
pubmed:
22
9
2019
medline:
19
3
2020
Statut:
ppublish
Résumé
Type 1 diabetes is the prototypical CD4 T cell-mediated autoimmune disease. Its genetic linkage to a single polymorphism at position 57 of the HLA class II DQβ chain makes it unique to study the molecular link between HLA and disease. However, investigating this relationship has been limited by a series of anatomical barriers, the small size and dispersion of the insulin-producing organ, and the scarcity of appropriate techniques and reagents to interrogate antigen-specific CD4 T cells both in man and rodent models. Over the past few years, single-cell technologies, paired with new biostatistical methods, have changed this landscape. Using these tools, we have identified the first molecular link between MHC class II and the onset of type 1 diabetes. The translation of these observations to man is within reach using similar approaches and the lessons learned from rodent models.
Identifiants
pubmed: 31540941
pii: 68/10/1886
doi: 10.2337/dbi18-0064
pmc: PMC6754240
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
1886-1891Subventions
Organisme : NCATS NIH HHS
ID : KL2 TR001112
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK117138
Pays : United States
Organisme : NCATS NIH HHS
ID : TL1 TR002551
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR002550
Pays : United States
Informations de copyright
© 2019 by the American Diabetes Association.
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