Quantitative and rapid Plasmodium falciparum malaria diagnosis and artemisinin-resistance detection using a CMOS Lab-on-Chip platform.
CMOS
ISFET
LAMP
Lab-on-Chip
Malaria
P. falciparum
Point-of-Care
SNP
Journal
Biosensors & bioelectronics
ISSN: 1873-4235
Titre abrégé: Biosens Bioelectron
Pays: England
ID NLM: 9001289
Informations de publication
Date de publication:
01 Dec 2019
01 Dec 2019
Historique:
received:
26
05
2019
revised:
01
08
2019
accepted:
04
09
2019
pubmed:
22
9
2019
medline:
14
3
2020
entrez:
22
9
2019
Statut:
ppublish
Résumé
Early and accurate diagnosis of malaria and drug-resistance is essential to effective disease management. Available rapid malaria diagnostic tests present limitations in analytical sensitivity, drug-resistance testing and/or quantification. Conversely, diagnostic methods based on nucleic acid amplification stepped forwards owing to their high sensitivity, specificity and robustness. Nevertheless, these methods commonly rely on optical measurements and complex instrumentation which limit their applicability in resource-poor, point-of-care settings. This paper reports the specific, quantitative and fully-electronic detection of Plasmodium falciparum, the predominant malaria-causing parasite worldwide, using a Lab-on-Chip platform developed in-house. Furthermore, we demonstrate on-chip detection of C580Y, the most prevalent single-nucleotide polymorphism associated to artemisinin-resistant malaria. Real-time non-optical DNA sensing is facilitated using Ion-Sensitive Field-Effect Transistors, fabricated in unmodified complementary metal-oxide-semiconductor (CMOS) technology, coupled with loop-mediated isothermal amplification. This work holds significant potential for the development of a fully portable and quantitative malaria diagnostic that can be used as a rapid point-of-care test.
Identifiants
pubmed: 31541787
pii: S0956-5663(19)30757-2
doi: 10.1016/j.bios.2019.111678
pmc: PMC7224984
mid: EMS86402
pii:
doi:
Substances chimiques
Artemisinins
0
artemisinin
9RMU91N5K2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
111678Subventions
Organisme : Wellcome Trust
ID : 100993
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_PC_16046
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/N012275/1
Pays : United Kingdom
Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.
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