The effects of growth factors on proliferation of spermatogonial stem cells from Guangxi Bama mini-pig.
Adult Germline Stem Cells
/ cytology
Animals
Cell Line
Cell Proliferation
/ drug effects
China
Coculture Techniques
Fibroblast Growth Factor 2
/ pharmacology
Glial Cell Line-Derived Neurotrophic Factor
/ pharmacology
Glial Cell Line-Derived Neurotrophic Factor Receptors
/ pharmacology
Male
Mice
Spermatogenesis
Swine
Swine, Miniature
Testis
/ cytology
Transcription Factors
/ metabolism
growth factors
male germline stem cells
mini-pig
proliferation
Journal
Reproduction in domestic animals = Zuchthygiene
ISSN: 1439-0531
Titre abrégé: Reprod Domest Anim
Pays: Germany
ID NLM: 9015668
Informations de publication
Date de publication:
Dec 2019
Dec 2019
Historique:
received:
13
06
2019
revised:
10
09
2019
accepted:
16
09
2019
pubmed:
24
9
2019
medline:
21
5
2020
entrez:
24
9
2019
Statut:
ppublish
Résumé
The objective of this study was to investigate the effects of different growth factors on the proliferation of Bama mini-pig spermatogonial stem cells (SSCs) in vitro. The growth factors glial cell line-derived neurotrophic factor (GDNF), leukaemia inhibitory factor (LIF), GDNF family receptor alpha-1 (GFRα1) and basic fibroblast growth factor (bFGF) were investigated. The SSCs were seeded on SIM mouse embryo-derived thioguanine- and ouabain-resistant (STO) feeder layers. Cultivation of the cells were subjected to a factorial design of the growth factors GDNF + bFGF, GDNF + bFGF + GFRα1, LIF + bFGF and LIF + bFGF + GFRα1. The SSCs could propagate for 25 passages in the medium adding GDNF + bFGF + GFRα1, 22 passages in the medium adding GDNF + bFGF, 6 passages in the medium adding LIF + bFGF, or LIF + bFGF + GFRα1. qRT-PCR analysis showed that the highest mRNA expression levels of NANOG, POU5F, DDX4, GFRα1 and UCHL1 were detected in the group adding GDNF + bFGF + GFRα1. The SSCs from the group adding GDNF + bFGF + GFRα1 also showed UCHL1-, DBA- and CDH1-positive staining. Moreover, Stra8 and Scp3 expression, and haploid peak were detected after induction of the SSCs from the group adding GDNF + bFGF + GFRα1. In conclusion, pig SSCs could be maintained for long term in the presence of GDNF, bFGF, and GFRα1.
Substances chimiques
Glial Cell Line-Derived Neurotrophic Factor
0
Glial Cell Line-Derived Neurotrophic Factor Receptors
0
Transcription Factors
0
Fibroblast Growth Factor 2
103107-01-3
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1574-1582Subventions
Organisme : National Natural Science Foundation of China
ID : 31260277
Organisme : Guangxi High Education Innovation Research Group and Eminent Scholar Project
Informations de copyright
© 2019 Blackwell Verlag GmbH.
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