An arbitrary-spectrum spatial visual stimulator for vision research.

color vision dichromatic vision mouse neuroscience retina tetrachromatic vision two-photon calcium imaging visual system zebrafish

Journal

eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614

Informations de publication

Date de publication:
23 09 2019
Historique:
received: 24 05 2019
accepted: 20 09 2019
pubmed: 24 9 2019
medline: 6 2 2020
entrez: 24 9 2019
Statut: epublish

Résumé

Visual neuroscientists require accurate control of visual stimulation. However, few stimulator solutions simultaneously offer high spatio-temporal resolution and free control over the spectra of the light sources, because they rely on off-the-shelf technology developed for human trichromatic vision. Importantly, consumer displays fail to drive UV-shifted short wavelength-sensitive photoreceptors, which strongly contribute to visual behaviour in many animals, including mice, zebrafish and fruit flies. Moreover, many non-mammalian species feature more than three spectral photoreceptor types. Here, we present a flexible, spatial visual stimulator with up to six arbitrary spectrum chromatic channels. It combines a standard digital light processing engine with open source hard- and software that can be easily adapted to the experimentalist's needs. We demonstrate the capability of this general visual stimulator experimentally in the in vitro mouse retinal whole-mount and the in vivo zebrafish. With this work, we intend to start a community effort of sharing and developing a common stimulator design for vision research.

Identifiants

pubmed: 31545172
doi: 10.7554/eLife.48779
pii: 48779
pmc: PMC6783264
doi:
pii:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/R014817/1
Pays : United Kingdom
Organisme : Leverhulme Trust
ID : PLP-2017-005
Pays : International
Organisme : European Commission
ID : ERC-StG 'NeuroVisEco' 677687
Pays : International
Organisme : Horizon 2020 Framework Programme
ID : Marie Skłodowska-Curie grant agreement No 674901
Pays : International
Organisme : Bundesministerium für Bildung und Forschung
ID : FKZ: 01GQ1002
Pays : International
Organisme : Max-Planck-Gesellschaft
ID : M.FE.A.KYBE0004
Pays : International
Organisme : Deutsche Forschungsgemeinschaft
ID : Projektnummer 276693517 - SFB 1233
Pays : International
Organisme : Medical Research Council
ID : MC_PC_15071
Pays : United Kingdom

Informations de copyright

© 2019, Franke et al.

Déclaration de conflit d'intérêts

KF, AM, ZZ, MZ, PB, YQ, KS, TB, TE No competing interests declared

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Auteurs

Katrin Franke (K)

Institute for Ophthalmic Research, University of Tübingen, Tübingen, Germany.
Bernstein Center for Computational Neuroscience, University of Tübingen, Tübingen, Germany.

André Maia Chagas (A)

Institute for Ophthalmic Research, University of Tübingen, Tübingen, Germany.
Center for Integrative Neuroscience, University of Tübingen, Tübingen, Germany.
Sussex Neuroscience, School of Life Sciences, University of Sussex, Falmer, United Kingdom.

Zhijian Zhao (Z)

Institute for Ophthalmic Research, University of Tübingen, Tübingen, Germany.
Center for Integrative Neuroscience, University of Tübingen, Tübingen, Germany.

Maxime Jy Zimmermann (MJ)

Sussex Neuroscience, School of Life Sciences, University of Sussex, Falmer, United Kingdom.

Philipp Bartel (P)

Sussex Neuroscience, School of Life Sciences, University of Sussex, Falmer, United Kingdom.

Yongrong Qiu (Y)

Institute for Ophthalmic Research, University of Tübingen, Tübingen, Germany.
Center for Integrative Neuroscience, University of Tübingen, Tübingen, Germany.

Klaudia P Szatko (KP)

Institute for Ophthalmic Research, University of Tübingen, Tübingen, Germany.
Bernstein Center for Computational Neuroscience, University of Tübingen, Tübingen, Germany.

Tom Baden (T)

Institute for Ophthalmic Research, University of Tübingen, Tübingen, Germany.
Sussex Neuroscience, School of Life Sciences, University of Sussex, Falmer, United Kingdom.

Thomas Euler (T)

Institute for Ophthalmic Research, University of Tübingen, Tübingen, Germany.
Bernstein Center for Computational Neuroscience, University of Tübingen, Tübingen, Germany.
Center for Integrative Neuroscience, University of Tübingen, Tübingen, Germany.

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Classifications MeSH