Sample Preservation and Storage Significantly Impact Taxonomic and Functional Profiles in Metaproteomics Studies of the Human Gut Microbiome.
RNAlater
flash freezing
metagenomics
metaproteomics
microbiome
microbiota
proteomics
sample storage
Journal
Microorganisms
ISSN: 2076-2607
Titre abrégé: Microorganisms
Pays: Switzerland
ID NLM: 101625893
Informations de publication
Date de publication:
19 Sep 2019
19 Sep 2019
Historique:
received:
29
08
2019
revised:
13
09
2019
accepted:
17
09
2019
entrez:
25
9
2019
pubmed:
25
9
2019
medline:
25
9
2019
Statut:
epublish
Résumé
With the technological advances of the last decade, it is now feasible to analyze microbiome samples, such as human stool specimens, using multi-omic techniques. Given the inherent sample complexity, there exists a need for sample methods which preserve as much information as possible about the biological system at the time of sampling. Here, we analyzed human stool samples preserved and stored using different methods, applying metagenomics as well as metaproteomics. Our results demonstrate that sample preservation and storage have a significant effect on the taxonomic composition of identified proteins. The overall identification rates, as well as the proportion of proteins from
Identifiants
pubmed: 31546776
pii: microorganisms7090367
doi: 10.3390/microorganisms7090367
pmc: PMC6780314
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Fonds National de la Recherche Luxembourg
ID : C15/BN/10404093
Organisme : Deutsche Forschungsgemeinschaft
ID : FZT118
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