Novel Polyclonal Antibody Raised against Tetrodotoxin Using Its Haptenic Antigen Prepared from 4,9-anhydrotetrodotoxin Reacted with 1,2-Ethaneditiol and Further Reacted with Keyhole Limpet Hemocyanin.

Animals Antibodies / immunology Antigens / immunology Enzyme-Linked Immunosorbent Assay / methods Haptens / immunology Hemocyanins / immunology Rabbits Tetrodotoxin / analogs & derivatives
ELISA polyclonal antibody pufferfish tetrodotoxin tetrodotoxin analogs

Journal

Toxins
ISSN: 2072-6651
Titre abrégé: Toxins (Basel)
Pays: Switzerland
ID NLM: 101530765

Informations de publication

Date de publication:
20 09 2019
Historique:
received: 30 07 2019
revised: 18 09 2019
accepted: 19 09 2019
entrez: 25 9 2019
pubmed: 25 9 2019
medline: 21 10 2020
Statut: epublish

Résumé

A novel polyclonal antibody against tetrodotoxin (TTX) was raised using its haptenic antigen, where 4,9-anhydroTTX was reacted with 1,2-ethanedithiol and this derivative was further reacted with keyhole limpet hemocyanin (KLH). This newly designed antigen (KLH-TTX) was inoculated into rabbits, resulting in the production of the specific polyclonal antibody, which reacted well with TTX and its analogs, 4-

Identifiants

DOI: 10.3390/toxins11100551 PMID: 31547067 PMC: PMC6832204
pubmed: 31547067
pii: toxins11100551
doi: 10.3390/toxins11100551
pmc: PMC6832204
pii:
doi:

Substances chimiques

Antibodies 0
Antigens 0
Haptens 0
Tetrodotoxin 4368-28-9
Hemocyanins 9013-72-3
keyhole-limpet hemocyanin FV4Y0JO2CX

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Déclaration de conflit d'intérêts

The authors declare no conflict of interest.

Références

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Auteurs

Shigeru Sato (S)

School of Marine Biosciences, Kitasato University, Sagamihara-shi, Kanagawa 252-0373, Japan. shigeru@kitasato-u.ac.jp.

Suzuka Takaishi (S)

School of Marine Biosciences, Kitasato University, Sagamihara-shi, Kanagawa 252-0373, Japan. mf16004@st.kitasato-u.ac.jp.

Ko Yasumoto (K)

School of Marine Biosciences, Kitasato University, Sagamihara-shi, Kanagawa 252-0373, Japan. yasumoto@kitasato-u.ac.jp.

Shugo Watabe (S)

School of Marine Biosciences, Kitasato University, Sagamihara-shi, Kanagawa 252-0373, Japan. swatabe@kitasato-u.ac.jp.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Novel Polyclonal Antibody Raised against...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › Novel Polyclonal Antibody Raised against...
questionsmedicales.fr › Facteurs biologiques › Antigènes › Novel Polyclonal Antibody Raised against...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Dosage immunologique › Techniques d'immunoadsorption › Test ELISA › Novel Polyclonal Antibody Raised against...
questionsmedicales.fr › Facteurs biologiques › Antigènes › Épitopes › Haptènes › Novel Polyclonal Antibody Raised against...
questionsmedicales.fr › Facteurs biologiques › Pigments biologiques › Hémocyanine › Novel Polyclonal Antibody Raised against...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Lagomorpha › Lapins › Novel Polyclonal Antibody Raised against...
questionsmedicales.fr › Facteurs biologiques › Toxines biologiques › Toxines de la flore et de la faune marines › Tétrodotoxine › Novel Polyclonal Antibody Raised against...