A novel construct with biomechanical flexibility for articular cartilage regeneration.
BMSCs
Cartilage regeneration
Hydrostatic pressure
Mechanobiology
PRF
Journal
Stem cell research & therapy
ISSN: 1757-6512
Titre abrégé: Stem Cell Res Ther
Pays: England
ID NLM: 101527581
Informations de publication
Date de publication:
23 09 2019
23 09 2019
Historique:
received:
19
06
2019
accepted:
26
08
2019
revised:
13
08
2019
entrez:
25
9
2019
pubmed:
25
9
2019
medline:
28
7
2020
Statut:
epublish
Résumé
Although tissue-engineered cartilage has been broadly studied, complete integration of regenerated cartilage with residual cartilage is still difficult for the inferior mechanical and biochemical feature of neocartilage. Chondrogenesis of mesenchymal stem cells can be induced by biophysical and biochemical factors. In this study, autologous platelet-rich fibrin (PRF) membrane was used as a growth factor-rich scaffold that may facilitate differentiation of the transplanted bone marrow mesenchymal stem cells (BMSCs). At the same time, hydrostatic pressure was adopted for pre-adjustment of the seed cells before transplantation that may promote the mechanical flexibility of neocartilage. An in vitro study showed that the feasible hydrostatic pressure stimulation substantially promoted the chondrogenic potential of in vitro-cultured BMSC/PRF construct. In vivo results revealed that at every time point, the newborn tissues were the most favorable in the pressure-pretreated BMSC/PRF transplant group. Besides, the transplantation of feasible hydrostatic pressure-pretreated construct by BMSC sheet fragments and PRF granules could obviously improve the integration between the regenerated cartilage and host cartilage milieu, and thereby achieve boundaryless repair between the neocartilage and residual host cartilage tissue in rabbit temporomandibular joints. It could be concluded that feasible hydrostatic pressure may effectively promote the proliferation and chondrogenic differentiation of BMSCs in a BMSC/PRF construct. This newly formed construct with biomechanical flexibility showed a superior capacity for cartilage regeneration by promoting the mechanical properties and integration of neocartilage.
Sections du résumé
BACKGROUND
Although tissue-engineered cartilage has been broadly studied, complete integration of regenerated cartilage with residual cartilage is still difficult for the inferior mechanical and biochemical feature of neocartilage. Chondrogenesis of mesenchymal stem cells can be induced by biophysical and biochemical factors.
METHODS
In this study, autologous platelet-rich fibrin (PRF) membrane was used as a growth factor-rich scaffold that may facilitate differentiation of the transplanted bone marrow mesenchymal stem cells (BMSCs). At the same time, hydrostatic pressure was adopted for pre-adjustment of the seed cells before transplantation that may promote the mechanical flexibility of neocartilage.
RESULTS
An in vitro study showed that the feasible hydrostatic pressure stimulation substantially promoted the chondrogenic potential of in vitro-cultured BMSC/PRF construct. In vivo results revealed that at every time point, the newborn tissues were the most favorable in the pressure-pretreated BMSC/PRF transplant group. Besides, the transplantation of feasible hydrostatic pressure-pretreated construct by BMSC sheet fragments and PRF granules could obviously improve the integration between the regenerated cartilage and host cartilage milieu, and thereby achieve boundaryless repair between the neocartilage and residual host cartilage tissue in rabbit temporomandibular joints. It could be concluded that feasible hydrostatic pressure may effectively promote the proliferation and chondrogenic differentiation of BMSCs in a BMSC/PRF construct.
CONCLUSION
This newly formed construct with biomechanical flexibility showed a superior capacity for cartilage regeneration by promoting the mechanical properties and integration of neocartilage.
Identifiants
pubmed: 31547887
doi: 10.1186/s13287-019-1399-2
pii: 10.1186/s13287-019-1399-2
pmc: PMC6757433
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
298Références
Stem Cells. 2004;22(3):313-23
pubmed: 15153608
Curr Opin Cell Biol. 2009 Oct;21(5):630-5
pubmed: 19615877
Biomaterials. 2016 Aug;98:1-22
pubmed: 27177218
Arch Oral Biol. 2014 Oct;59(10):1108-18
pubmed: 25033382
Biomaterials. 2013 Jul;34(22):5506-20
pubmed: 23639531
Wound Repair Regen. 2004 May-Jun;12(3):337-45
pubmed: 15225212
J Biosci Bioeng. 2007 Dec;104(6):506-9
pubmed: 18215638
J Biomech Eng. 2015 Feb 1;137(2):020901
pubmed: 25322349
In Vitro Cell Dev Biol Anim. 2016 Mar;52(3):356-364
pubmed: 26822434
Cartilage. 2012 Jul;3(3):205-21
pubmed: 26069634
J Bone Joint Surg Br. 2009 May;91(5):565-76
pubmed: 19407287
Mech Dev. 2000 Jun;94(1-2):13-24
pubmed: 10842055
Biomaterials. 2000 Jan;21(1):49-61
pubmed: 10619678
Nat Mater. 2010 Jan;9(1):82-8
pubmed: 19838182
J Cell Physiol. 2019 Aug;234(10):17946-17958
pubmed: 30834523
Cell Mol Life Sci. 2016 Mar;73(6):1173-94
pubmed: 26811234
Science. 2012 Nov 16;338(6109):917-21
pubmed: 23161992
PLoS One. 2013 Nov 21;8(11):e81362
pubmed: 24278427
Biomed Mater. 2015 Sep 21;10(5):055011
pubmed: 26391756
Biomaterials. 2012 Nov;33(33):8395-405
pubmed: 22922021
Exp Hematol. 2004 Dec;32(12):1238-45
pubmed: 15588948
Tissue Eng Part A. 2010 Jun;16(6):1913-23
pubmed: 20088686
Mol Cell Biomech. 2011 Mar;8(1):43-60
pubmed: 21391327
J Biol Chem. 2003 Dec 19;278(51):50940-8
pubmed: 12952976
J Oral Maxillofac Surg. 2008 Sep;66(9):1804-11
pubmed: 18718386
Am J Sports Med. 2008 Nov;36(11):2091-9
pubmed: 18801942
Stem Cells. 2010 Nov;28(11):1992-6
pubmed: 20882533
J Bone Joint Surg Am. 1994 Apr;76(4):579-92
pubmed: 8150826
Artif Organs. 2001 Mar;25(3):172-9
pubmed: 11284883
Biomed Pharmacother. 2017 Sep;93:1165-1174
pubmed: 28738525
Arch Oral Biol. 2007 Feb;52(2):173-81
pubmed: 17056002
Life Sci. 2006 Apr 18;78(21):2480-7
pubmed: 16325208
Acta Biomater. 2012 Jul;8(6):2153-9
pubmed: 22426136
Biorheology. 2009;46(1):45-55
pubmed: 19252227
FASEB J. 2018 Oct;32(10):5298-5311
pubmed: 29688810
Plast Reconstr Surg. 2005 Oct;116(5):1453-60
pubmed: 16217495
Osteoarthritis Cartilage. 2013 Oct;21(10):1605-12
pubmed: 23916685
Biomech Model Mechanobiol. 2007 Jan;6(1-2):113-25
pubmed: 16691412
Stem Cell Res Ther. 2017 Dec 13;8(1):281
pubmed: 29237506
Knee Surg Sports Traumatol Arthrosc. 2008 Oct;16(10):896-903
pubmed: 18592218
Ann Biomed Eng. 2009 Mar;37(3):492-502
pubmed: 19125331
J Bone Miner Metab. 2005;23(3):219-25
pubmed: 15838624
Colloids Surf B Biointerfaces. 2017 Nov 1;159:838-848
pubmed: 28888201
Lancet. 2010 Aug 7;376(9739):440-8
pubmed: 20692530
Int J Oral Maxillofac Surg. 2011 Jul;40(7):704-9
pubmed: 21459556
Cells Tissues Organs. 2012;195(5):400-13
pubmed: 21832815
Biochem Biophys Res Commun. 2008 Dec 12;377(2):458-462
pubmed: 18851955
Artif Organs. 2006 Aug;30(8):586-96
pubmed: 16911312
Stem Cells Int. 2017;2017:5804147
pubmed: 28740513
Int J Mol Sci. 2019 May 27;20(10):
pubmed: 31137788
Arch Biochem Biophys. 2008 Jun 1;474(1):102-8
pubmed: 18375197
Tissue Eng Part B Rev. 2009 Mar;15(1):43-53
pubmed: 19196119
Int J Mol Sci. 2018 Aug 11;19(8):
pubmed: 30103493
J Am Acad Orthop Surg. 2013 May;21(5):303-11
pubmed: 23637149
Osteoarthritis Cartilage. 2003 Jan;11(1):55-64
pubmed: 12505488
Arkh Anat Gistol Embriol. 1969 Mar;56(3):3-11
pubmed: 4903779
Int J Exp Pathol. 2012 Dec;93(6):389-400
pubmed: 23075006
PLoS One. 2016 Jul 29;11(7):e0160121
pubmed: 27472059
Proc Natl Acad Sci U S A. 2016 Dec 13;113(50):14360-14365
pubmed: 27911782
Scand J Plast Reconstr Surg Hand Surg. 1999 Sep;33(3):341-4
pubmed: 10505451
Birth Defects Res C Embryo Today. 2013 Sep;99(3):192-202
pubmed: 24078496
J Control Release. 2008 Apr 7;127(1):12-21
pubmed: 18280603
Osteoarthritis Cartilage. 2013 May;21(5):646-54
pubmed: 23485933
Curr Osteoporos Rep. 2010 Jun;8(2):98-104
pubmed: 20425617
Osteoarthritis Cartilage. 2009 Nov;17(11):1408-15
pubmed: 19477310
J Craniomaxillofac Surg. 2011 Jul;39(5):380-6
pubmed: 20810288
Cell Tissue Res. 2012 Jan;347(1):21-36
pubmed: 21643690