Regenerative potential of human dental pulp stem cells in the treatment of stress urinary incontinence: In vitro and in vivo study.
Journal
Cell proliferation
ISSN: 1365-2184
Titre abrégé: Cell Prolif
Pays: England
ID NLM: 9105195
Informations de publication
Date de publication:
Nov 2019
Nov 2019
Historique:
received:
12
05
2019
revised:
10
07
2019
accepted:
16
07
2019
pubmed:
26
9
2019
medline:
21
12
2019
entrez:
26
9
2019
Statut:
ppublish
Résumé
To evaluate the regenerative potential of human dental pulp stem cells (hDPSCs) in an animal model of stress urinary incontinence (SUI). SUI, an involuntary leakage of urine, is due to physical stress involving an increase in bladder pressure and a damage of external urethral sphincter affecting muscles and nerves. Conventional therapies can only relieve the symptoms. Human DPSCs are characterized by peculiar stemness and immunomodulatory properties and might provide an alternative tool for SUI therapy. In vitro phase: hDPSCs were induced towards the myogenic commitment following a 24 hours pre-conditioning with 5-aza-2'-deoxycytidine (5-Aza), then differentiation was evaluated. In vivo phase: pudendal nerve was transected in female rats to induce stress urinary incontinence; then, pre-differentiated hDPSCs were injected in the striated urethral sphincter. Four weeks later, urethral sphincter regeneration was assayed through histological, functional and immunohistochemical analyses. Human DPSCs were able to commit towards myogenic lineage in vitro and, four weeks after cell injection, hDPSCs engrafted in the external urethral sphincter whose thickness was almost recovered, committed towards myogenic lineage in vivo, promoted vascularization and an appreciable recovery of the continence. Moreover, hDPSCs were detected within the nerve, suggesting their participation in repair of transected nerve. These promising data and further investigations on immunomodulatory abilities of hDPSCs would allow to make them a potential tool for alternative therapies of SUI.
Identifiants
pubmed: 31553127
doi: 10.1111/cpr.12675
pmc: PMC6868931
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e12675Subventions
Organisme : UNIMORE
ID : 81711
Informations de copyright
© 2019 The Authors. Cell Proliferation Published by John Wiley & Sons Ltd.
Références
Neurourol Urodyn. 2004;23(2):166-71
pubmed: 14983430
Neurourol Urodyn. 2014 Jun;33(5):507-10
pubmed: 23797972
Obstet Gynecol Int. 2012;2012:612946
pubmed: 21941558
BJU Int. 2016 Jul;118(1):20-34
pubmed: 26991606
Acta Cir Bras. 2013;28 Suppl 1:37-42
pubmed: 23381822
Stem Cells Transl Med. 2014 Aug;3(8):936-41
pubmed: 24985079
Int Urogynecol J. 2013 May;24(5):759-62
pubmed: 22940842
Int Urogynecol J Pelvic Floor Dysfunct. 2008 Jun;19(6):881-3
pubmed: 18204978
J Urol. 2011 Mar;185(3):1132-8
pubmed: 21255803
Urology. 2009 Jun;73(6):1203-9
pubmed: 19362350
J Urol. 2010 Mar;183(3):1232-40
pubmed: 20096880
J Urol. 2005 May;173(5):1647-53
pubmed: 15821528
Health Technol Assess. 2010 Aug;14(40):1-188, iii-iv
pubmed: 20738930
Neurourol Urodyn. 2008;27(8):749-57
pubmed: 18951445
BJU Int. 2008 Sep;102(7):878-84
pubmed: 18489526
Cochrane Database Syst Rev. 2014 Dec 17;(12):CD001756
pubmed: 25517397
J Urol. 2011 Jan;185(1):219-23
pubmed: 21074790
Int J Urol. 2016 Jul;23(7):581-6
pubmed: 27062371
Lancet. 1999 Jul;354 Suppl 1:SI32-4
pubmed: 10437854
Minerva Urol Nefrol. 2019 Jun;71(3):230-239
pubmed: 30547904
Int Urogynecol J. 2013 Jun;24(6):913-9
pubmed: 23224022
J Tissue Eng Regen Med. 2018 Feb;12(2):e774-e785
pubmed: 27943583
Cell Prolif. 2019 Nov;52(6):e12675
pubmed: 31553127
BMC Med. 2012 Aug 21;10:94
pubmed: 22906045
Cell Transplant. 2012;21(5):959-70
pubmed: 22449414
Urol Int. 2015;95(4):417-21
pubmed: 26043913
Int Urogynecol J Pelvic Floor Dysfunct. 2000;11(1):15-7
pubmed: 10738929
Stem Cell Res Ther. 2018 Sep 21;9(1):239
pubmed: 30241573
Am J Obstet Gynecol. 2013 Sep;209(3):244.e1-9
pubmed: 23659987
Clinics (Sao Paulo). 2016 Feb;71(2):94-100
pubmed: 26934239
Stem Cell Res Ther. 2013 May 21;4(3):53
pubmed: 23688855
Cell Transplant. 2014;23(11):1395-406
pubmed: 23866688
Stem Cell Res Ther. 2015 Aug 28;6:156
pubmed: 26316011
J Am Geriatr Soc. 2010 Aug;58(8):1504-11
pubmed: 20653804
J Urol. 2006 Apr;175(4):1408-10
pubmed: 16516009
Cochrane Database Syst Rev. 2012 Jan 18;1:CD003195
pubmed: 22258952
JAMA. 2000 Jan 19;283(3):354-60
pubmed: 10647798
Radiology. 2008 Nov;249(2):534-40
pubmed: 18796659
Cytotherapy. 2010;12(1):88-95
pubmed: 19878076
Biomaterials. 2014 Nov;35(34):9311-21
pubmed: 25145852
Womens Health (Lond). 2014 Mar;10(2):201-17
pubmed: 24601811
Tissue Eng. 2005 Jul-Aug;11(7-8):1034-44
pubmed: 16144439
J Korean Med Sci. 2010 May;25(5):663-70
pubmed: 20436699
J Cell Mol Med. 2014 Mar;18(3):434-43
pubmed: 24460735
IUBMB Life. 2018 Oct;70(10):976-984
pubmed: 30212601
Int Urogynecol J Pelvic Floor Dysfunct. 2007 Apr;18(4):397-400
pubmed: 16804634
J Urol. 2013 Feb;189(2):595-601
pubmed: 23260547
Cochrane Database Syst Rev. 2009 Oct 07;(4):CD002912
pubmed: 19821297
Front Physiol. 2018 May 28;9:547
pubmed: 29892229
Int J Urol. 2014 Mar;21(3):294-300
pubmed: 24033774
Urology. 2010 Mar;75(3):718-23
pubmed: 19969332
Rev Urol. 2004;6 Suppl 3:S3-9
pubmed: 16985863
Urol Int. 2011;86(1):110-6
pubmed: 20689260
PLoS One. 2012;7(11):e50542
pubmed: 23209773
Eur Urol. 2005 Sep;48(3):488-94
pubmed: 15967568
BMC Dev Biol. 2015 Mar 14;15:14
pubmed: 25879198
Eur Urol. 2007 Sep;52(3):868-74
pubmed: 17412488