Selective activation of serotoninergic dorsal raphe neurons facilitates sleep through anxiolysis.
AAV
EEG/EMG
anxiety
chemogenetics
dorsal raphe
mice
serotonin
stress
thermoregulation
Journal
Sleep
ISSN: 1550-9109
Titre abrégé: Sleep
Pays: United States
ID NLM: 7809084
Informations de publication
Date de publication:
13 02 2020
13 02 2020
Historique:
received:
06
05
2019
revised:
18
08
2019
pubmed:
26
9
2019
medline:
15
4
2021
entrez:
26
9
2019
Statut:
ppublish
Résumé
A role for the brain's serotoninergic (5HT) system in the regulation of sleep and wakefulness has been long suggested. Yet, previous studies employing pharmacological, lesion and genetically driven approaches have produced inconsistent findings, leaving 5HT's role in sleep-wake regulation incompletely understood. Here we sought to define the specific contribution of 5HT neurons within the dorsal raphe nucleus (DRN5HT) to sleep and arousal control. To do this, we employed a chemogenetic strategy to selectively and acutely activate DRN5HT neurons and monitored sleep-wake using electroencephalogram recordings. We additionally assessed indices of anxiety using the open field and elevated plus maze behavioral tests and employed telemetric-based recordings to test effects of acute DRN5HT activation on body temperature and locomotor activity. Our findings indicate that the DRN5HT cell population may not modulate sleep-wake per se, but rather that its activation has apparent anxiolytic properties, suggesting the more nuanced view that DRN5HT neurons are sleep permissive under circumstances that produce anxiety or stress.
Identifiants
pubmed: 31553451
pii: 5573750
doi: 10.1093/sleep/zsz231
pmc: PMC7315407
pii:
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NHLBI NIH HHS
ID : P01 HL149630
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS073613
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS092652
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS103161
Pays : United States
Informations de copyright
© Sleep Research Society 2019. Published by Oxford University Press on behalf of the Sleep Research Society. All rights reserved. For permissions, please e-mail journals.permissions@oup.com.
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