Ciliogenesis-coupled accumulation of IFT-B proteins in a novel cytoplasmic compartment.
Journal
Genes to cells : devoted to molecular & cellular mechanisms
ISSN: 1365-2443
Titre abrégé: Genes Cells
Pays: England
ID NLM: 9607379
Informations de publication
Date de publication:
Nov 2019
Nov 2019
Historique:
received:
25
07
2019
revised:
18
09
2019
accepted:
18
09
2019
pubmed:
26
9
2019
medline:
17
3
2020
entrez:
26
9
2019
Statut:
ppublish
Résumé
Cluap1/IFT38 is a ciliary protein that belongs to the IFT-B complex and is required for ciliogenesis. In this study, we have examined the behaviors of Cluap1 protein in nonciliated and ciliated cells. In proliferating cells, Cluap1 is located at the distal appendage of the mother centriole. When cells are induced to form cilia, Cluap1 is found in a novel noncentriolar compartment, the cytoplasmic IFT spot, which mainly exists once in a cell. Other IFT-B proteins such as IFT46 and IFT88 are colocalized in this spot. The cytoplasmic IFT spot is present in mouse embryonic fibroblasts (MEFs) but is absent in ciliogenesis-defective MEFs lacking Cluap1, Kif3a or Odf2. The cytoplasmic IFT spot is also found in mouse embryos but is absent in the Cluap1 mutant embryo. When MEFs are induced to form cilia, the cytoplasmic IFT spot appears at an early step of ciliogenesis but starts to disappear when ciliogenesis is mostly completed. These results suggest that IFT-B proteins such as Cluap1 accumulate in a previously undescribed cytoplasmic compartment during ciliogenesis.
Substances chimiques
Cluap1 protein, mouse
0
Cytoskeletal Proteins
0
Heat-Shock Proteins
0
IFT46 protein, mouse
0
Intracellular Signaling Peptides and Proteins
0
Kif3a protein, mouse
0
Odf2 protein, mouse
0
Tg737Rpw protein, mouse
0
Tumor Suppressor Proteins
0
Kinesins
EC 3.6.4.4
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
731-745Subventions
Organisme : Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan
ID : 14H01435
Organisme : Core Research for Evolutional Science and Technology (CREST) of the Japan Science and Technology Corporation
ID : JPMJCR13W5
Informations de copyright
© 2019 Molecular Biology Society of Japan and John Wiley & Sons Australia, Ltd.
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