Preservation of zebrafish genetic resources through testis cryopreservation and spermatogonia transplantation.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
25 09 2019
25 09 2019
Historique:
received:
27
07
2018
accepted:
24
08
2019
entrez:
27
9
2019
pubmed:
27
9
2019
medline:
3
11
2020
Statut:
epublish
Résumé
Zebrafish is one of the most commonly used model organisms in biomedical, developmental and genetic research. The production of several thousands of transgenic lines is leading to difficulties in maintaining valuable genetic resources as cryopreservation protocols for eggs and embryos are not yet developed. In this study, we utilized testis cryopreservation (through both slow-rate freezing and vitrification) and spermatogonia transplantation as effective methods for long-term storage and line reconstitution in zebrafish. During freezing, utilization of 1.3 M of dimethyl sulfoxide (Me
Identifiants
pubmed: 31554831
doi: 10.1038/s41598-019-50169-1
pii: 10.1038/s41598-019-50169-1
pmc: PMC6761286
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
13861Références
ACS Nano. 2017 Aug 22;11(8):7869-7878
pubmed: 28702993
Proc Natl Acad Sci U S A. 2005 Mar 15;102(11):4074-9
pubmed: 15728735
Mol Reprod Dev. 2017 Oct;84(10):1100-1111
pubmed: 28731265
Theriogenology. 2007 Jul 15;68(2):128-36
pubmed: 17544099
Mol Reprod Dev. 2016 Apr;83(4):298-311
pubmed: 26860442
Proc Natl Acad Sci U S A. 2013 Jan 29;110(5):1640-5
pubmed: 23319620
Cryobiology. 2016 Apr;72(2):165-8
pubmed: 26827783
Gen Comp Endocrinol. 2017 May 1;245:77-83
pubmed: 27401260
Anim Reprod Sci. 2014 Jan 30;144(3-4):129-37
pubmed: 24439024
Comp Biochem Physiol C Toxicol Pharmacol. 2009 Mar;149(2):224-32
pubmed: 18691673
Methods Mol Biol. 2009;546:45-65
pubmed: 19378097
Cryobiology. 2015 Dec;71(3):367-73
pubmed: 26408854
Sci Rep. 2017 Mar 03;7:43185
pubmed: 28256523
Comp Biochem Physiol Part D Genomics Proteomics. 2011 Mar;6(1):55-61
pubmed: 20541987
Proc Natl Acad Sci U S A. 2002 Nov 12;99(23):14919-24
pubmed: 12397179
Biotechniques. 2003 Nov;35(5):956-8, 960, 962 passim
pubmed: 14628669
Biol Reprod. 1999 Nov;61(5):1331-9
pubmed: 10529282
Cryobiology. 2018 Aug;83:75-83
pubmed: 29852130
PLoS One. 2019 Apr 18;14(4):e0205481
pubmed: 30998742
Dev Biol. 2008 Dec 15;324(2):277-87
pubmed: 18930041
J Vis Exp. 2018 Mar 4;(133):
pubmed: 29553568
Sci Rep. 2015 Nov 02;5:16045
pubmed: 26522018
Theriogenology. 2019 Jul 15;133:216-227
pubmed: 31155037
Gen Comp Endocrinol. 2017 May 1;245:69-76
pubmed: 27318279
Cryobiology. 2016 Oct;73(2):286-90
pubmed: 27496280
Biochem Biophys Res Commun. 2016 Sep 23;478(3):1478-83
pubmed: 27581197
Stem Cell Reports. 2015 Jan 13;4(1):61-73
pubmed: 25434820
Gen Comp Endocrinol. 2017 May 1;245:102-107
pubmed: 27174751
Anim Reprod Sci. 2012 Oct;134(3-4):197-202
pubmed: 22921390
Dev Cell. 2017 Dec 18;43(6):704-715.e5
pubmed: 29257950
Cryobiology. 2017 Jun;76:154-157
pubmed: 28438562
Hum Reprod. 2008 Oct;23(10):2256-65
pubmed: 18614614
PLoS One. 2014 Dec 22;9(12):e115574
pubmed: 25531401
Zebrafish. 2018 Jun;15(3):279-290
pubmed: 29369744
Zebrafish. 2016 Jul;13 Suppl 1:S30-8
pubmed: 27031282
Nature. 2013 Apr 25;496(7446):498-503
pubmed: 23594743