Use of GelMA for 3D printing of cardiac myocytes and fibroblasts.
3D bioprinting
GelMA
bioluminescence imaging
cardiac fibroblasts
cardiomyocytes
cell viability
Journal
Journal of 3D printing in medicine
ISSN: 2059-4763
Titre abrégé: J 3D Print Med
Pays: England
ID NLM: 101716116
Informations de publication
Date de publication:
Mar 2019
Mar 2019
Historique:
entrez:
27
9
2019
pubmed:
27
9
2019
medline:
27
9
2019
Statut:
ppublish
Résumé
To 3D print heart tissue, one must understand how the main two types of cardiac cells are affected by the printing process. Effects of gelatin methacryloyl (GelMA) concentration, extruder pressure and duration of UV exposure on survival of cardiac myocytes and fibroblasts were examined using lactate dehydrogenase and LIVE/DEAD assays, bioluminescence imaging and morphological assessment. Cell survival within 3D printed cardiomyocyte-laden GelMA constructs was more sensitive to extruder pressure and GelMA concentrations than within 3D fibroblast-laden GelMA constructs. Cells within both types of constructs were adversely impacted by the UV curing step. Use of mixed cell populations and enrichment of bioink formulation with fibronectin led to an improvement of cardiomyocyte survival and spreading.
Identifiants
pubmed: 31555480
doi: 10.2217/3dp-2018-0017
pmc: PMC6760315
mid: NIHMS1011886
doi:
Types de publication
Journal Article
Langues
eng
Pagination
11-22Subventions
Organisme : American Heart Association-American Stroke Association
ID : 14GRNT20380806
Pays : United States
Organisme : NHLBI NIH HHS
ID : R21 HL122882
Pays : United States
Références
J Biomater Sci Polym Ed. 2000;11(5):439-57
pubmed: 10896041
Circ Res. 2002 Feb 8;90(2):223-30
pubmed: 11834716
Annu Rev Biomed Eng. 2002;4:235-60
pubmed: 12117758
Am J Physiol Heart Circ Physiol. 2002 Aug;283(2):H741-9
pubmed: 12124223
Am J Physiol Heart Circ Physiol. 2003 Dec;285(6):H2531-42
pubmed: 12893638
Biomaterials. 2005 Apr;26(11):1211-8
pubmed: 15475050
Cardiovasc Res. 2005 Jan 1;65(1):40-51
pubmed: 15621032
Biophys J. 2005 Oct;89(4):2332-49
pubmed: 16055545
J Biomed Mater Res A. 2008 Sep;86(3):713-24
pubmed: 18041719
Biomaterials. 2010 Jul;31(21):5536-44
pubmed: 20417964
J Tissue Eng Regen Med. 2011 Jun;5(6):e115-25
pubmed: 21604379
Sensors (Basel). 2011;11(1):180-206
pubmed: 22346573
Macromol Biosci. 2013 May;13(5):551-61
pubmed: 23420700
Biomaterials. 2013 Jul;34(23):5813-20
pubmed: 23642535
Biomaterials. 2014 Jan;35(1):49-62
pubmed: 24112804
Adv Drug Deliv Rev. 2016 Jan 15;96:135-55
pubmed: 26232525
Adv Funct Mater. 2015 Feb 11;25(6):977-986
pubmed: 26327819
Adv Drug Deliv Rev. 2016 Jan 15;96:234-244
pubmed: 26428619
Sci Adv. 2015 Sep 25;1(8):e1500655
pubmed: 26601274
Sci Adv. 2015 Oct 23;1(9):e1500758
pubmed: 26601312
Biotechnol Adv. 2016 Jul-Aug;34(4):422-434
pubmed: 26724184
Methods Mol Biol. 2016;1408:303-17
pubmed: 26965132
Physiol Genomics. 2016 Oct 1;48(10):762-770
pubmed: 27614203
Tissue Eng Part B Rev. 2017 Oct;23(5):451-461
pubmed: 28067115
JACC Cardiovasc Imaging. 2017 Feb;10(2):171-184
pubmed: 28183437
Acta Biomater. 2017 Jun;55:120-130
pubmed: 28455218
Adv Healthc Mater. 2017 Jun;6(12):
pubmed: 28464555
Materials (Basel). 2016 Sep 24;9(10):null
pubmed: 28773918
Biomater Sci. 2017 Sep 26;5(10):2093-2105
pubmed: 28805830
Biofabrication. 2018 Jan 12;10(2):024102
pubmed: 29176035
ACS Appl Mater Interfaces. 2018 Feb 28;10(8):6849-6857
pubmed: 29405059
Science. 1997 May 30;276(5317):1425-8
pubmed: 9162012