Flotillins promote T cell receptor sorting through a fast Rab5-Rab11 endocytic recycling axis.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
26 09 2019
26 09 2019
Historique:
received:
31
07
2018
accepted:
28
08
2019
entrez:
28
9
2019
pubmed:
29
9
2019
medline:
7
1
2020
Statut:
epublish
Résumé
The targeted endocytic recycling of the T cell receptor (TCR) to the immunological synapse is essential for T cell activation. Despite this, the mechanisms that underlie the sorting of internalised receptors into recycling endosomes remain poorly understood. To build a comprehensive picture of TCR recycling during T cell activation, we developed a suite of new imaging and quantification tools centred on photoactivation of fluorescent proteins. We show that the membrane-organising proteins, flotillin-1 and -2, are required for TCR to reach Rab5-positive endosomes immediately after endocytosis and for transfer from Rab5- to Rab11a-positive compartments. We further observe that after sorting into in Rab11a-positive vesicles, TCR recycles to the plasma membrane independent of flotillin expression. Our data suggest a mechanism whereby flotillins delineate a fast Rab5-Rab11a endocytic recycling axis and functionally contribute to regulate the spatial organisation of these endosomes.
Identifiants
pubmed: 31558725
doi: 10.1038/s41467-019-12352-w
pii: 10.1038/s41467-019-12352-w
pmc: PMC6763463
doi:
Substances chimiques
Membrane Proteins
0
Receptors, Antigen, T-Cell
0
flotillins
0
Green Fluorescent Proteins
147336-22-9
rab11 protein
EC 3.6.1.-
rab GTP-Binding Proteins
EC 3.6.5.2
rab5 GTP-Binding Proteins
EC 3.6.5.2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4392Références
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