A 'parameiosis' drives depolyploidization and homologous recombination in Candida albicans.
Candida albicans
/ genetics
Candidiasis
/ microbiology
Chromosomes, Fungal
/ genetics
DNA Damage
Diploidy
Endodeoxyribonucleases
/ genetics
Fungal Proteins
/ genetics
Homologous Recombination
/ genetics
Humans
Meiosis
/ genetics
Mitosis
/ genetics
Rec A Recombinases
/ genetics
Recombinational DNA Repair
Tetraploidy
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
26 09 2019
26 09 2019
Historique:
received:
10
12
2018
accepted:
28
08
2019
entrez:
28
9
2019
pubmed:
29
9
2019
medline:
7
1
2020
Statut:
epublish
Résumé
Meiosis is a conserved tenet of sexual reproduction in eukaryotes, yet this program is seemingly absent from many extant species. In the human fungal pathogen Candida albicans, mating of diploid cells generates tetraploid products that return to the diploid state via a non-meiotic process of depolyploidization known as concerted chromosome loss (CCL). Here, we report that recombination rates are more than three orders of magnitude higher during CCL than during normal mitotic growth. Furthermore, two conserved 'meiosis-specific' factors play central roles in CCL as SPO11 mediates DNA double-strand break formation while both SPO11 and REC8 regulate chromosome stability and promote inter-homolog recombination. Unexpectedly, SPO11 also promotes DNA repair and recombination during normal mitotic divisions. These results indicate that C. albicans CCL represents a 'parameiosis' that blurs the conventional boundaries between mitosis and meiosis. They also reveal parallels with depolyploidization in mammalian cells and provide potential insights into the evolution of meiosis.
Identifiants
pubmed: 31558727
doi: 10.1038/s41467-019-12376-2
pii: 10.1038/s41467-019-12376-2
pmc: PMC6763455
doi:
Substances chimiques
Fungal Proteins
0
Rec A Recombinases
EC 2.7.7.-
Endodeoxyribonucleases
EC 3.1.-
meiotic recombination protein SPO11
EC 3.1.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4388Subventions
Organisme : NIAID NIH HHS
ID : R01 AI141893
Pays : United States
Organisme : NIDCR NIH HHS
ID : F31 DE023726
Pays : United States
Organisme : NIAID NIH HHS
ID : R21 AI122011
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI081704
Pays : United States
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