Multiplex gene expression profile in inflamed mucosa of patients with Crohn's disease ileal localization: A pilot study.
Colon
Crohn’s disease
Ileum
Inflammation
Messenger ribonucleic acid
Microbiota
Th1/Th17
Journal
World journal of clinical cases
ISSN: 2307-8960
Titre abrégé: World J Clin Cases
Pays: United States
ID NLM: 101618806
Informations de publication
Date de publication:
06 Sep 2019
06 Sep 2019
Historique:
received:
20
03
2019
revised:
12
07
2019
accepted:
27
07
2019
entrez:
28
9
2019
pubmed:
29
9
2019
medline:
29
9
2019
Statut:
ppublish
Résumé
Crohn's disease (CD) is a complex disorder resulting from the interaction of genetic, environmental, and microbial factors. The pathogenic process may potentially affect any segment of the gastrointestinal tract, but a selective location in the terminal ileum was reported in 50% of patients. To characterize clinical sub-phenotypes (colonic and/or ileal) within the same disease, in order to identify new therapeutic targets. 14 consecutive patients undergoing surgery for ileal CD were recruited for this study. Peripheral blood samples from each patient were collected and the main polymorphisms of the gene We found a significant increase of Th17 (IL17A and IL17F, IL 23R and CCR6) and Th1 (IFN-γ) gene expression in inflamed mucosa compared to non-inflamed sites of 14 CD patients. We observed that the expression of ileal genes related to Th1 and Th17 activity is strongly activated as well as the expression of genes involved in microbiota regulation.
Sections du résumé
BACKGROUND
BACKGROUND
Crohn's disease (CD) is a complex disorder resulting from the interaction of genetic, environmental, and microbial factors. The pathogenic process may potentially affect any segment of the gastrointestinal tract, but a selective location in the terminal ileum was reported in 50% of patients.
AIM
OBJECTIVE
To characterize clinical sub-phenotypes (colonic and/or ileal) within the same disease, in order to identify new therapeutic targets.
METHODS
METHODS
14 consecutive patients undergoing surgery for ileal CD were recruited for this study. Peripheral blood samples from each patient were collected and the main polymorphisms of the gene
RESULTS
RESULTS
We found a significant increase of Th17 (IL17A and IL17F, IL 23R and CCR6) and Th1 (IFN-γ) gene expression in inflamed mucosa compared to non-inflamed sites of 14 CD patients.
CONCLUSION
CONCLUSIONS
We observed that the expression of ileal genes related to Th1 and Th17 activity is strongly activated as well as the expression of genes involved in microbiota regulation.
Identifiants
pubmed: 31559282
doi: 10.12998/wjcc.v7.i17.2463
pmc: PMC6745337
doi:
Types de publication
Journal Article
Langues
eng
Pagination
2463-2476Déclaration de conflit d'intérêts
Conflict-of-interest statement: The authors declare that they have no competing interests.
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