Novel role of Tieg1 in muscle metabolism and mitochondrial oxidative capacities.
Animals
DNA-Binding Proteins
/ genetics
Disease Models, Animal
Electron Transport Complex IV
/ metabolism
Female
Metabolome
Mice
Mice, Knockout
Mitochondria
/ metabolism
Muscle, Skeletal
/ metabolism
Muscles
/ metabolism
Oxidative Stress
/ physiology
Physical Conditioning, Animal
/ physiology
Succinate Dehydrogenase
/ metabolism
Transcription Factors
/ genetics
Klf10
Tieg1
metabolism
mitochondria
skeletal muscle
Journal
Acta physiologica (Oxford, England)
ISSN: 1748-1716
Titre abrégé: Acta Physiol (Oxf)
Pays: England
ID NLM: 101262545
Informations de publication
Date de publication:
03 2020
03 2020
Historique:
received:
07
11
2018
revised:
20
09
2019
accepted:
23
09
2019
pubmed:
29
9
2019
medline:
1
7
2021
entrez:
28
9
2019
Statut:
ppublish
Résumé
Tieg1 is involved in multiple signalling pathways, human diseases, and is highly expressed in muscle where its functions are poorly understood. We have utilized Tieg1 knockout (KO) mice to identify novel and important roles for this transcription factor in regulating muscle ultrastructure, metabolism and mitochondrial functions in the soleus and extensor digitorum longus (EDL) muscles. RNA sequencing, immunoblotting, transmission electron microscopy, MRI, NMR, histochemical and mitochondrial function assays were performed. Loss of Tieg1 expression resulted in altered sarcomere organization and a significant decrease in mitochondrial number. Histochemical analyses demonstrated an absence of succinate dehydrogenase staining and a decrease in cytochrome c oxidase (COX) enzyme activity in KO soleus with similar, but diminished, effects in the EDL. Decreased complex I, COX and citrate synthase (CS) activities were detected in the soleus muscle of KO mice indicating altered mitochondrial function. Complex I activity was also diminished in KO EDL. Significant decreases in CS and respiratory chain complex activities were identified in KO soleus. Our findings have implicated novel roles for Tieg1 in muscle including regulation of gene expression, metabolic activity and organization of tissue ultrastructure. This muscle phenotype resembles diseases associated with exercise intolerance and myopathies of unknown consequence.
Substances chimiques
DNA-Binding Proteins
0
Tieg1 protein, mouse
0
Transcription Factors
0
Succinate Dehydrogenase
EC 1.3.99.1
Electron Transport Complex IV
EC 1.9.3.1
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e13394Subventions
Organisme : NIH Clinical Center
ID : R01 DE14036
Pays : International
Informations de copyright
© 2019 Scandinavian Physiological Society. Published by John Wiley & Sons Ltd.
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