Tick-borne encephalitis virus inhibits rRNA synthesis and host protein production in human cells of neural origin.
Journal
PLoS neglected tropical diseases
ISSN: 1935-2735
Titre abrégé: PLoS Negl Trop Dis
Pays: United States
ID NLM: 101291488
Informations de publication
Date de publication:
09 2019
09 2019
Historique:
received:
27
03
2019
accepted:
03
09
2019
revised:
09
10
2019
pubmed:
29
9
2019
medline:
15
1
2020
entrez:
28
9
2019
Statut:
epublish
Résumé
Tick-borne encephalitis virus (TBEV), a member of the genus Flavivirus (Flaviviridae), is a causative agent of a severe neuroinfection. Recently, several flaviviruses have been shown to interact with host protein synthesis. In order to determine whether TBEV interacts with this host process in its natural target cells, we analysed de novo protein synthesis in a human cell line derived from cerebellar medulloblastoma (DAOY HTB-186). We observed a significant decrease in the rate of host protein synthesis, including the housekeeping genes HPRT1 and GAPDH and the known interferon-stimulated gene viperin. In addition, TBEV infection resulted in a specific decrease of RNA polymerase I (POLR1) transcripts, 18S and 28S rRNAs and their precursor, 45-47S pre-rRNA, but had no effect on the POLR3 transcribed 5S rRNA levels. To our knowledge, this is the first report of flavivirus-induced decrease of specifically POLR1 rRNA transcripts accompanied by host translational shut-off.
Identifiants
pubmed: 31560682
doi: 10.1371/journal.pntd.0007745
pii: PNTD-D-19-00498
pmc: PMC6785130
doi:
Substances chimiques
RNA Precursors
0
RNA, Ribosomal
0
RNA Polymerase I
EC 2.7.7.6
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0007745Subventions
Organisme : Medical Research Council
ID : MC_UU_12014/8
Pays : United Kingdom
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Cold Spring Harb Perspect Biol. 2013 Jan 01;5(1):a012351
pubmed: 23209131
Sci Rep. 2017 Dec 19;7(1):17761
pubmed: 29259342
BMC Bioinformatics. 2017 Nov 29;18(1):529
pubmed: 29187165
Clin Infect Dis. 1999 Apr;28(4):882-90
pubmed: 10825054
J Mol Biol. 2014 Mar 20;426(6):1210-9
pubmed: 24157441
J Biol Chem. 2011 Apr 22;286(16):14226-36
pubmed: 21385877
Chem Rev. 2018 Apr 25;118(8):4448-4482
pubmed: 29652486
Virology. 2009 Jul 20;390(1):139-50
pubmed: 19467556
Viruses. 2018 Jun 21;10(7):
pubmed: 29933625
Proc Natl Acad Sci U S A. 1998 Sep 15;95(19):11089-94
pubmed: 9736694
Acta Virol. 1975 Nov;19(6):498
pubmed: 1999
J Virol. 2014 Nov;88(21):12202-12
pubmed: 25122777
J Clin Invest. 2012 Dec;122(12):4447-60
pubmed: 23160199
J Gen Virol. 2017 Jan;98(1):2-3
pubmed: 28218572
J Neuroinflammation. 2016 Jan 27;13:22
pubmed: 26819220
Cell Microbiol. 2014 Jun;16(6):834-48
pubmed: 24245804
J Gen Virol. 1981 Dec;57(Pt 2):263-74
pubmed: 6172553
J Virol. 1993 Jun;67(6):3027-35
pubmed: 8098776
Curr Opin Struct Biol. 2017 Dec;47:88-94
pubmed: 28743025
BMC Cell Biol. 2008 Jun 17;9:32
pubmed: 18559082
Nat Rev Microbiol. 2008 Sep;6(9):699-708
pubmed: 18587411
Biochemistry. 2009 Jul 21;48(28):6571-84
pubmed: 19485420
J Gen Virol. 1996 May;77 ( Pt 5):1035-42
pubmed: 8609469
RNA. 2010 Jan;16(1):131-40
pubmed: 19934231
Nat Rev Microbiol. 2011 Oct 17;9(12):860-75
pubmed: 22002165
J Virol. 2017 Aug 24;91(18):
pubmed: 28659485
Viruses. 2016 Apr 16;8(4):102
pubmed: 27092522
Virology. 1973 Feb;51(2):454-65
pubmed: 4632654
Sci Rep. 2017 Nov 30;7(1):16652
pubmed: 29192272
J Virol. 2000 Oct;74(19):8793-802
pubmed: 10982321
Vaccine. 2003 Apr 1;21 Suppl 1:S1-2
pubmed: 12628808
J Natl Cancer Inst. 1973 Nov;51(5):1417-23
pubmed: 4357758
Viruses. 2016 Jun 02;8(6):
pubmed: 27271653
J Virol. 2018 Mar 14;92(7):
pubmed: 29321318
PLoS One. 2014 Jul 24;9(7):e103264
pubmed: 25058476
Braz J Med Biol Res. 2009 Nov;42(11):1020-6
pubmed: 19855901
DNA Cell Biol. 2018 Sep;37(9):725-730
pubmed: 30059238
J Virol. 2006 Mar;80(6):2976-86
pubmed: 16501107
Vaccine. 2003 Apr 1;21 Suppl 1:S11-8
pubmed: 12628810
MBio. 2017 Jan 10;8(1):
pubmed: 28074025
Lek List. 1954 Jan 1;9(1):3-5
pubmed: 13131921
Antiviral Res. 2003 Jan;57(1-2):129-46
pubmed: 12615309
J Gen Virol. 1982 Oct;62 (Pt 2):271-85
pubmed: 6292351
Virus Genes. 2017 Dec;53(6):807-813
pubmed: 28733876
J Gen Virol. 2017 Aug;98(8):2043-2060
pubmed: 28786780
Curr Opin Virol. 2014 Dec;9:134-42
pubmed: 25462445
Nat Rev Mol Cell Biol. 2018 Jul;19(7):464-478
pubmed: 29740129
Viruses. 2019 May 21;11(5):null
pubmed: 31117224
J Gen Virol. 1997 May;78 ( Pt 5):1049-57
pubmed: 9152422
Bull World Health Organ. 1969;40(1):113-21
pubmed: 4183812
Biomed Res Int. 2014;2014:308371
pubmed: 25110671
J Neuropathol Exp Neurol. 1985 Sep;44(5):472-85
pubmed: 2993532
J Virol. 2009 Apr;83(8):3637-46
pubmed: 19193790
Proc Natl Acad Sci U S A. 2007 May 22;104(21):9041-6
pubmed: 17502609
Biotechnol Genet Eng Rev. 2018 Apr;34(1):60-75
pubmed: 29804514
Antimicrob Agents Chemother. 1977 Jul;12(1):11-5
pubmed: 883813
Cell Stress. 2018 May 10;2(6):125-140
pubmed: 31225478
Sci Rep. 2017 Jun 30;7(1):4475
pubmed: 28667332
Front Microbiol. 2016 Aug 25;7:1233
pubmed: 27610098
Adv Virus Res. 2003;59:177-228
pubmed: 14696330
Ticks Tick Borne Dis. 2019 Jun;10(4):868-874
pubmed: 31047827
Virology. 2008 Aug 15;378(1):118-22
pubmed: 18572216
J Virol. 2018 Mar 14;92(7):
pubmed: 29321322
Wiley Interdiscip Rev RNA. 2015 Mar-Apr;6(2):225-42
pubmed: 25346433
J Neuropathol Exp Neurol. 2005 Jun;64(6):506-12
pubmed: 15977642
Viral Immunol. 2004;17(4):498-515
pubmed: 15671747