Gene expression profiling of skeletal myogenesis in human embryonic stem cells reveals a potential cascade of transcription factors regulating stages of myogenesis, including quiescent/activated satellite cell-like gene expression.
Cell Differentiation
/ drug effects
Cell Line
Gene Expression Profiling
Gene Expression Regulation, Developmental
Glycogen Synthase Kinase 3 beta
/ antagonists & inhibitors
Human Embryonic Stem Cells
/ metabolism
Humans
Muscle Development
/ genetics
Muscle, Skeletal
/ cytology
Pyridines
/ pharmacology
Pyrimidines
/ pharmacology
Satellite Cells, Skeletal Muscle
/ metabolism
Transcription Factors
/ metabolism
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2019
2019
Historique:
received:
21
05
2019
accepted:
10
09
2019
entrez:
28
9
2019
pubmed:
29
9
2019
medline:
3
4
2020
Statut:
epublish
Résumé
Human embryonic stem cell (hESC)-derived skeletal muscle progenitors (SMP)-defined as PAX7-expressing cells with myogenic potential-can provide an abundant source of donor material for muscle stem cell therapy. As in vitro myogenesis is decoupled from in vivo timing and 3D-embryo structure, it is important to characterize what stage or type of muscle is modeled in culture. Here, gene expression profiling is analyzed in hESCs over a 50 day skeletal myogenesis protocol and compared to datasets of other hESC-derived skeletal muscle and adult murine satellite cells. Furthermore, day 2 cultures differentiated with high or lower concentrations of CHIR99021, a GSK3A/GSK3B inhibitor, were contrasted. Expression profiling of the 50 day time course identified successively expressed gene subsets involved in mesoderm/paraxial mesoderm induction, somitogenesis, and skeletal muscle commitment/formation which could be regulated by a putative cascade of transcription factors. Initiating differentiation with higher CHIR99021 concentrations significantly increased expression of MSGN1 and TGFB-superfamily genes, notably NODAL, resulting in enhanced paraxial mesoderm and reduced ectoderm/neuronal gene expression. Comparison to adult satellite cells revealed that genes expressed in 50-day cultures correlated better with those expressed by quiescent or early activated satellite cells, which have the greatest therapeutic potential. Day 50 cultures were similar to other hESC-derived skeletal muscle and both expressed known and novel SMP surface proteins. Overall, a putative cascade of transcription factors has been identified which regulates four stages of myogenesis. Subsets of these factors were upregulated by high CHIR99021 or their binding sites were significantly over-represented during SMP activation, ranging from quiescent to late-activated stages. This analysis serves as a resource to further study the progression of in vitro skeletal myogenesis and could be mined to identify novel markers of pluripotent-derived SMPs or regulatory transcription/growth factors. Finally, 50-day hESC-derived SMPs appear similar to quiescent/early activated satellite cells, suggesting they possess therapeutic potential.
Identifiants
pubmed: 31560727
doi: 10.1371/journal.pone.0222946
pii: PONE-D-19-14400
pmc: PMC6764674
doi:
Substances chimiques
Chir 99021
0
Pyridines
0
Pyrimidines
0
Transcription Factors
0
GSK3B protein, human
EC 2.7.11.1
Glycogen Synthase Kinase 3 beta
EC 2.7.11.1
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0222946Subventions
Organisme : NIAMS NIH HHS
ID : R01 AR044031
Pays : United States
Organisme : CIHR
ID : MOP-119458
Pays : Canada
Organisme : CIHR
ID : FDN-148387
Pays : Canada
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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