Histone Acetyltransferase-Dependent Pathways Mediate Upregulation of NADPH Oxidase 5 in Human Macrophages under Inflammatory Conditions: A Potential Mechanism of Reactive Oxygen Species Overproduction in Atherosclerosis.
Atherosclerosis
/ enzymology
E1A-Associated p300 Protein
/ genetics
Epigenesis, Genetic
Histone Acetyltransferases
/ genetics
Histones
/ biosynthesis
Humans
Lipopolysaccharides
/ pharmacology
Macrophages
/ drug effects
NADPH Oxidase 5
/ biosynthesis
Reactive Oxygen Species
/ metabolism
THP-1 Cells
Transfection
Up-Regulation
Journal
Oxidative medicine and cellular longevity
ISSN: 1942-0994
Titre abrégé: Oxid Med Cell Longev
Pays: United States
ID NLM: 101479826
Informations de publication
Date de publication:
2019
2019
Historique:
received:
27
03
2019
revised:
14
06
2019
accepted:
04
07
2019
entrez:
1
10
2019
pubmed:
1
10
2019
medline:
21
3
2020
Statut:
epublish
Résumé
Histone acetylation plays a major role in epigenetic regulation of gene expression. Monocyte-derived macrophages express functional NADPH oxidase 5 (Nox5) that contributes to oxidative stress in atherogenesis. The mechanisms of Nox5 regulation are not entirely elucidated. The aim of this study was to investigate the expression pattern of key histone acetyltransferase subtypes (p300, HAT1) in human atherosclerosis and to determine their role in mediating the upregulation of Nox5 in macrophages under inflammatory conditions. Human nonatherosclerotic and atherosclerotic tissue samples were collected in order to determine the expression of p300 and HAT1 isoforms, H3K27ac, and Nox5. In vitro determinations were done on human macrophages exposed to lipopolysaccharide in the absence or presence of histone acetyltransferase inhibitors. Western blot, immunohistochemistry, immunofluorescence, real-time PCR, transfection, and chromatin immunoprecipitation assay were employed. The protein levels of p300 and HAT1 isoforms, H3K27ac, and Nox5 were found significantly elevated in human atherosclerotic specimens. Immunohistochemistry/immunofluorescence staining revealed that p300, HAT1, H3K27ac, H3K9ac, and Nox5 proteins were colocalized in the area of CD45
Identifiants
pubmed: 31565149
doi: 10.1155/2019/3201062
pmc: PMC6745143
doi:
Substances chimiques
Histones
0
Lipopolysaccharides
0
Reactive Oxygen Species
0
NADPH Oxidase 5
EC 1.6.3.-
NOX5 protein, human
EC 1.6.3.-
E1A-Associated p300 Protein
EC 2.3.1.48
EP300 protein, human
EC 2.3.1.48
Histone Acetyltransferases
EC 2.3.1.48
histone acetyltransferase type B complex
EC 2.3.1.48
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
3201062Informations de copyright
Copyright © 2019 Mihaela-Loredana Vlad et al.
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
Références
Circ Res. 2010 Apr 30;106(8):1363-73
pubmed: 20339118
Circulation. 2002 Mar 26;105(12):1429-35
pubmed: 11914250
Cell Tissue Res. 2010 Dec;342(3):325-39
pubmed: 21052718
J Cell Mol Med. 2014 Jul;18(7):1467-77
pubmed: 24797079
Diabetes. 2017 Oct;66(10):2691-2703
pubmed: 28747378
Clin Sci (Lond). 2016 Aug 1;130(15):1363-74
pubmed: 27190136
Br J Pharmacol. 2017 Jun;174(12):1533-1554
pubmed: 28332701
Nat Rev Drug Discov. 2006 Sep;5(9):769-84
pubmed: 16955068
Circ Res. 2018 Sep 14;123(7):773-786
pubmed: 30355081
Blood. 2017 Oct 12;130(15):1734-1745
pubmed: 28830888
Redox Biol. 2015 Aug;5:358-366
pubmed: 26133261
J Am Coll Cardiol. 2008 Nov 25;52(22):1803-9
pubmed: 19022160
Curr Pharm Des. 2015;21(41):5999-6008
pubmed: 26510438
Biochem Biophys Res Commun. 2015 May 22;461(1):172-9
pubmed: 25871798
Clin Sci (Lond). 2017 May 1;131(10):981-990
pubmed: 28473473
Dis Model Mech. 2016 May 1;9(5):541-52
pubmed: 26989193
Eur Heart J. 2009 Feb;30(3):266-77
pubmed: 19147603
Trends Pharmacol Sci. 2015 Apr;36(4):226-35
pubmed: 25758254
Cardiovasc Pathol. 2016 Mar-Apr;25(2):79-86
pubmed: 26764138
Circ Res. 2010 May 14;106(9):1489-97
pubmed: 20360249
Free Radic Biol Med. 2016 Oct;99:167-178
pubmed: 27498117
Int J Biochem Cell Biol. 2016 Jun;75:123-30
pubmed: 27102411
Nat Commun. 2016 Nov 08;7:13436
pubmed: 27824038
Front Biosci (Schol Ed). 2012 Jan 01;4:651-70
pubmed: 22202083
Nat Med. 2016 Sep;22(9):1002-12
pubmed: 27455510
Redox Biol. 2018 Jun;16:332-343
pubmed: 29587244
J Am Soc Nephrol. 2014 Apr;25(4):784-97
pubmed: 24262797
Biochem Pharmacol. 2016 Feb 15;102:130-140
pubmed: 26718586
Atherosclerosis. 2014 Nov;237(1):208-19
pubmed: 25244505
Nucleic Acids Res. 2001 May 1;29(9):e45
pubmed: 11328886
EMBO J. 2001 Mar 15;20(6):1331-40
pubmed: 11250899
J Biol Chem. 2001 Nov 30;276(48):44502-3
pubmed: 11577095
PLoS One. 2012;7(10):e48326
pubmed: 23118986
Circulation. 2014 Mar 11;129(10):1139-51
pubmed: 24396039
J Lipid Res. 2009 Apr;50 Suppl:S277-81
pubmed: 18987388
Trends Mol Med. 2017 Apr;23(4):332-347
pubmed: 28291707
Oxid Med Cell Longev. 2014;2014:641979
pubmed: 24693336
Cardiovasc Res. 2011 Jun 1;90(3):413-20
pubmed: 21233251
Toxicol Lett. 2009 Dec 15;191(2-3):195-202
pubmed: 19735705
J Cell Mol Med. 2016 Oct;20(10):1932-44
pubmed: 27297729
Free Radic Biol Med. 2012 May 1;52(9):1497-507
pubmed: 22348975
Circulation. 2002 Mar 12;105(10):1158-61
pubmed: 11889007