Relaxin-3 receptor (RXFP3) activation in the nucleus of the solitary tract modulates respiratory rate and the arterial chemoreceptor reflex in rat.
Animals
Chemoreceptor Cells
/ drug effects
Intercellular Signaling Peptides and Proteins
/ pharmacology
Microinjections
/ methods
Organ Culture Techniques
Rats
Rats, Sprague-Dawley
Receptors, G-Protein-Coupled
/ agonists
Receptors, Peptide
/ agonists
Respiratory Rate
/ drug effects
Solitary Nucleus
/ drug effects
Arterial chemoreceptor reflex
Brainstem
Breathing control
Nucleus of the solitary tract
Relaxin-3
Journal
Respiratory physiology & neurobiology
ISSN: 1878-1519
Titre abrégé: Respir Physiol Neurobiol
Pays: Netherlands
ID NLM: 101140022
Informations de publication
Date de publication:
01 2020
01 2020
Historique:
received:
23
09
2019
revised:
26
09
2019
accepted:
27
09
2019
pubmed:
1
10
2019
medline:
6
6
2020
entrez:
1
10
2019
Statut:
ppublish
Résumé
The neuropeptide relaxin-3 is expressed by the pontine nucleus incertus. Relaxin-3 and synthetic agonist peptides modulate arousal and cognitive processes via activation of the relaxin-family peptide 3 receptor (RXFP3). Despite the presence of RXFP3 in the nucleus of the solitary tract (NTS), the ability of RXFP3 to modulate NTS-mediated cardiorespiratory functions has not been explored. Therefore, we examined the effects of bilateral microinjections of the selective agonist, RXFP3-A2 (40 μM, 100 nL/side), into the NTS in perfused working-heart-brainstem-preparations from rats (n = 6), while recording phrenic, vagal, and thoracic sympathetic chain activity (PNA, VNA, t-SCA) and heart rate (HR). RXFP3-A2 significantly increased respiratory rate and shortened post-inspiratory VNA. RXFP3-A2 in the NTS also significantly enhanced arterial chemoreceptor reflex (a-CR)-mediated tachypnea. However, RXFP3-A2 had no significant effect on HR and t-SCA at baseline or during the a-CR. These data represent the first evidence that RXFP3 activation in the NTS can selectively modulate respiration at baseline and during reflex behaviour.
Identifiants
pubmed: 31568840
pii: S1569-9048(19)30354-4
doi: 10.1016/j.resp.2019.103310
pii:
doi:
Substances chimiques
Intercellular Signaling Peptides and Proteins
0
RXFP3 protein, rat
0
RXFP3-a2 peptide
0
Receptors, G-Protein-Coupled
0
Receptors, Peptide
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
103310Informations de copyright
Copyright © 2019. Published by Elsevier B.V.