In Vivo Microdialysis of Endogenous and

13C-labeled succinate LC-MS cerebral ischemia endothelin-1 energy metabolism malonate micro-dialysis mitochondrial dysfunction reperfusion tricarboxylic acid cycle

Journal

Metabolites
ISSN: 2218-1989
Titre abrégé: Metabolites
Pays: Switzerland
ID NLM: 101578790

Informations de publication

Date de publication:
27 Sep 2019
Historique:
received: 31 07 2019
revised: 24 09 2019
accepted: 25 09 2019
entrez: 2 10 2019
pubmed: 2 10 2019
medline: 2 10 2019
Statut: epublish

Résumé

Cerebral micro-dialysis allows continuous sampling of extracellular metabolites, including glucose, lactate and pyruvate. Transient ischemic events cause a rapid drop in glucose and a rise in lactate levels. Following such events, the lactate/pyruvate (L/P) ratio may remain elevated for a prolonged period of time. In neurointensive care clinics, this ratio is considered a metabolic marker of ischemia and/or mitochondrial dysfunction. Here we propose a novel, sensitive microdialysis liquid chromatography-mass spectrometry (LC-MS) approach to monitor mitochondrial dysfunction in living brain using perfusion with

Identifiants

pubmed: 31569792
pii: metabo9100204
doi: 10.3390/metabo9100204
pmc: PMC6835622
pii:
doi:

Types de publication

Journal Article

Langues

eng

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Auteurs

Jesper F Havelund (JF)

VILLUM Center for Bioanalytical Sciences, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark. jhav@bmb.sdu.dk.

Kevin H Nygaard (KH)

Department of Neurosurgery, Odense University Hospital, University of Southern Denmark, Sdr. Boulevard 29, 5000 Odense C, Denmark. kevin.nygaard@hotmail.com.
BRIDGE-Brain ResearchE-Inter-Disciplinary Guided Excellence, Institute of Clinical Research, University of Southern Denmark, Winsløwparken 19, 5000 Odense C, Denmark. kevin.nygaard@hotmail.com.

Troels H Nielsen (TH)

Department of Neurosurgery, Odense University Hospital, University of Southern Denmark, Sdr. Boulevard 29, 5000 Odense C, Denmark. troels.nielsen@rsyd.dk.
BRIDGE-Brain ResearchE-Inter-Disciplinary Guided Excellence, Institute of Clinical Research, University of Southern Denmark, Winsløwparken 19, 5000 Odense C, Denmark. troels.nielsen@rsyd.dk.

Carl-Henrik Nordström (CH)

Department of Neurosurgery, Odense University Hospital, University of Southern Denmark, Sdr. Boulevard 29, 5000 Odense C, Denmark. carl-henrik.nordstrom@med.lu.se.
BRIDGE-Brain ResearchE-Inter-Disciplinary Guided Excellence, Institute of Clinical Research, University of Southern Denmark, Winsløwparken 19, 5000 Odense C, Denmark. carl-henrik.nordstrom@med.lu.se.

Frantz R Poulsen (FR)

Department of Neurosurgery, Odense University Hospital, University of Southern Denmark, Sdr. Boulevard 29, 5000 Odense C, Denmark. frantz.r.poulsen@rsyd.dk.
BRIDGE-Brain ResearchE-Inter-Disciplinary Guided Excellence, Institute of Clinical Research, University of Southern Denmark, Winsløwparken 19, 5000 Odense C, Denmark. frantz.r.poulsen@rsyd.dk.

Nils J Færgeman (NJ)

VILLUM Center for Bioanalytical Sciences, Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, 5230 Odense M, Denmark. nils.f@bmb.sdu.dk.

Axel Forsse (A)

Department of Neurosurgery, Odense University Hospital, University of Southern Denmark, Sdr. Boulevard 29, 5000 Odense C, Denmark. axel.forsse@gmail.com.
BRIDGE-Brain ResearchE-Inter-Disciplinary Guided Excellence, Institute of Clinical Research, University of Southern Denmark, Winsløwparken 19, 5000 Odense C, Denmark. axel.forsse@gmail.com.

Jan Bert Gramsbergen (JB)

Institute of Molecular Medicine, University of Southern Denmark, 55, 5230 Odense C, Denmark. jbgramsbergen@health.sdu.dk.

Classifications MeSH