Leader cell PLCγ1 activation during keratinocyte collective migration is induced by EGFR localization and clustering.
immobilized growth factor
receptor clustering
re‐epithelialization
wound healing
Journal
Bioengineering & translational medicine
ISSN: 2380-6761
Titre abrégé: Bioeng Transl Med
Pays: United States
ID NLM: 101689146
Informations de publication
Date de publication:
Sep 2019
Sep 2019
Historique:
received:
14
04
2019
revised:
14
06
2019
accepted:
17
06
2019
entrez:
2
10
2019
pubmed:
2
10
2019
medline:
2
10
2019
Statut:
epublish
Résumé
Re-epithelialization is a critical step in wound healing and results from the collective migration of keratinocytes. Previous work demonstrated that immobilized, but not soluble, epidermal growth factor (EGF) resulted in leader cell-specific activation of phospholipase C gamma 1 (PLCγ1) in HaCaT keratinocytes, and that this PLCγ1 activation was necessary to drive persistent cell migration. To determine the mechanism responsible for wound edge-localized PLCγ1 activation, we examined differences in cell area, cell-cell interactions, and EGF receptor (EGFR) localization between wound edge and bulk cells treated with vehicle, soluble EGF, or immobilized EGF. Our results support a multistep mechanism where EGFR translocation from the lateral membrane to the basolateral/basal membrane allows clustering in response to immobilized EGF. This analysis of factors regulating PLCγ1 activation is a crucial step toward developing therapies or wound dressings capable of modulating this signal and, consequently, cell migration.
Identifiants
pubmed: 31572796
doi: 10.1002/btm2.10138
pii: BTM210138
pmc: PMC6764804
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e10138Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM099031
Pays : United States
Informations de copyright
© 2019 The Authors. Bioengineering & Translational Medicine published by Wiley Periodicals, Inc. on behalf of The American Institute of Chemical Engineers.
Déclaration de conflit d'intérêts
The authors declare no competing financial interests.
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