A bioinspired 3D shape olibanum-collagen-gelatin scaffolds with tunable porous microstructure for efficient neural tissue regeneration.
Animals
Boswellia
/ chemistry
Cell Proliferation
Cells, Cultured
Collagen
/ chemistry
Frankincense
/ chemistry
Gelatin
/ chemistry
Male
Mesenchymal Stem Cells
/ chemistry
Nerve Regeneration
Nerve Tissue
/ growth & development
Particle Size
Porosity
Rats
Rats, Sprague-Dawley
Surface Properties
Tissue Scaffolds
/ chemistry
collagen
freeze-cast
gelatin
neural
olibanum
scaffold
Journal
Biotechnology progress
ISSN: 1520-6033
Titre abrégé: Biotechnol Prog
Pays: United States
ID NLM: 8506292
Informations de publication
Date de publication:
01 2020
01 2020
Historique:
received:
22
12
2018
revised:
14
07
2019
accepted:
17
09
2019
pubmed:
3
10
2019
medline:
9
6
2021
entrez:
3
10
2019
Statut:
ppublish
Résumé
There are a number of procedures for regeneration of injured nerves; however, tissue engineering scaffolds seems to be a promising approach for recovery of the functionality of the injured nerves. Consequently, in this study, olibanum-collagen-gelatin scaffolds were fabricated by freeze-cast technology. For this purpose, the olibanum and collagen were extracted from natural sources. The effect of solidification gradient on microstructure and properties of scaffolds was investigated. Scanning electron microscopy micrographs showed the formation of lamellar-type microstructure in which the average pore size reduced with an increase in freezing rate. According to the results, the prepared scaffolds at lower freezing rate showed a slight reduction in mechanical strength while the swelling and biodegradation ratio were increased due to the presence of larger pores and unidirectional channels. The composition of scaffolds and oriented microstructure improved cellular interaction. In addition, scaffolds with lower freezing rate exhibited promising results in terms of adhesion, spreading, and proliferation. In brief, the synthesized scaffolds at lower solidification rate have the potential for more in vitro and in vivo analyses to regeneration of neural defects.
Substances chimiques
Gelatin
9000-70-8
Collagen
9007-34-5
Frankincense
R9XLF1R1WM
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2918Subventions
Organisme : Alborz University of Medical Sciences
Pays : International
Informations de copyright
© 2019 American Institute of Chemical Engineers.
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