Brodifacoum does not modulate human cannabinoid receptor-mediated hyperpolarization of AtT20 cells or inhibition of adenylyl cyclase in HEK 293 cells.
Cannabinoid receptor signaling
Overdose
Superwarfarin
Synthetic cannabinoid
Journal
PeerJ
ISSN: 2167-8359
Titre abrégé: PeerJ
Pays: United States
ID NLM: 101603425
Informations de publication
Date de publication:
2019
2019
Historique:
received:
27
03
2019
accepted:
23
08
2019
entrez:
4
10
2019
pubmed:
4
10
2019
medline:
4
10
2019
Statut:
epublish
Résumé
Synthetic cannabinoids are a commonly used class of recreational drugs that can have significant adverse effects. There have been sporadic reports of co-consumption of illicit drugs with rodenticides such as warfarin and brodifacoum (BFC) over the past 20 years but recently, hundreds of people have been reported to have been poisoned with a mixture of synthetic cannabinoids and BFC. We have sought to establish whether BFC directly affects cannabinoid receptors, or their activation by the synthetic cannabinoid CP55940 or the phytocannabinoid Δ The effects of BFC on the hyperpolarization of wild type AtT20 cells, or AtT20 cells stably expressing human CB BFC did not activate CB BFC is not a cannabinoid receptor agonist, and appeared not to affect cannabinoid receptor activation. Our data suggests there is no pharmacodynamic rationale for mixing BFC with synthetic cannabinoids; however, it does not speak to whether BFC may affect synthetic cannabinoid metabolism or biodistribution. The reasons underlying the mixing of BFC with synthetic cannabinoids are unknown, and it remains to be established whether the "contamination" was deliberate or accidental. However, the consequences for people who ingested the mixture were often serious, and sometimes fatal, but this seems unlikely to be due to BFC action at cannabinoid receptors.
Sections du résumé
BACKGROUND
BACKGROUND
Synthetic cannabinoids are a commonly used class of recreational drugs that can have significant adverse effects. There have been sporadic reports of co-consumption of illicit drugs with rodenticides such as warfarin and brodifacoum (BFC) over the past 20 years but recently, hundreds of people have been reported to have been poisoned with a mixture of synthetic cannabinoids and BFC. We have sought to establish whether BFC directly affects cannabinoid receptors, or their activation by the synthetic cannabinoid CP55940 or the phytocannabinoid Δ
METHODS
METHODS
The effects of BFC on the hyperpolarization of wild type AtT20 cells, or AtT20 cells stably expressing human CB
RESULTS
RESULTS
BFC did not activate CB
DISCUSSION
CONCLUSIONS
BFC is not a cannabinoid receptor agonist, and appeared not to affect cannabinoid receptor activation. Our data suggests there is no pharmacodynamic rationale for mixing BFC with synthetic cannabinoids; however, it does not speak to whether BFC may affect synthetic cannabinoid metabolism or biodistribution. The reasons underlying the mixing of BFC with synthetic cannabinoids are unknown, and it remains to be established whether the "contamination" was deliberate or accidental. However, the consequences for people who ingested the mixture were often serious, and sometimes fatal, but this seems unlikely to be due to BFC action at cannabinoid receptors.
Identifiants
pubmed: 31579608
doi: 10.7717/peerj.7733
pii: 7733
pmc: PMC6765355
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e7733Informations de copyright
©2019 Sachdev et al.
Déclaration de conflit d'intérêts
Mark Connor is an Academic Editor for PeerJ. None of the authors have any other competing interests.
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