HPLC-DAD-ESI-QTOF-MS/MS profiling of Zygophyllum album roots extract and assessment of its cardioprotective effect against deltamethrin-induced myocardial injuries in rat, by suppression of oxidative stress-related inflammation and apoptosis via NF-κB signaling pathway.
Animals
Anti-Inflammatory Agents
/ chemistry
Apoptosis
/ drug effects
Cardiotonic Agents
/ chemistry
Chromatography, High Pressure Liquid
Disease Models, Animal
Ethnopharmacology
Humans
Lipid Peroxidation
/ drug effects
Male
Myocardial Infarction
/ chemically induced
Myocardium
/ immunology
NF-kappa B
/ metabolism
Nitriles
/ toxicity
Oxidative Stress
/ drug effects
Plant Extracts
/ chemistry
Plant Roots
/ chemistry
Pyrethrins
/ toxicity
Rats
Rats, Sprague-Dawley
Signal Transduction
/ drug effects
Tandem Mass Spectrometry
Tunisia
Zygophyllum
/ chemistry
Deltamethrin
Fibrosis
HPLC-DAD-ESI-QTOF-MS
Myocardial
Oxidative stress
Zygophyllum album
Journal
Journal of ethnopharmacology
ISSN: 1872-7573
Titre abrégé: J Ethnopharmacol
Pays: Ireland
ID NLM: 7903310
Informations de publication
Date de publication:
30 Jan 2020
30 Jan 2020
Historique:
received:
01
04
2019
revised:
12
09
2019
accepted:
30
09
2019
pubmed:
4
10
2019
medline:
24
3
2020
entrez:
4
10
2019
Statut:
ppublish
Résumé
Zygophyllum album is widely used to treat many cardiovascular diseases (CVDs) and as anti-inflammatory plant. This study aimed to investigate the mechanism of the potential protective effects of Zygophyllum album roots extract (ZARE) against myocardial damage and fibrosis induced by a chronic exposure to deltamethrin (DLM) in rats. Bioactive compounds present in ZARE were analyzed by HPLC-DAD-ESI-QTOF-MS/MS. In vivo, DLM (4 mg/kg body weight), ZARE (400 mg/kg body weight) and DLM with ZARE were administered to rats orally for 60 days. Biochemical markers (LDH, ALT, CK, CK-MB and cTn-I) were assessed in the plasma by an auto-analyzer. Pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6) were evaluated by a sandwich ELISA. NF-κB was quantified at mRNA levels by real time PCR. Heart tissue was used to determine cardiac oxidative stress markers (MDA, PC, SOD, CAT, and GPx). Masson's Trichrome (MT) and Sirius Red (SR) stainings were used for explored fibrosis statues. Phytochemical analysis using HPLC-DAD-ESI-QTOF-MS/MS revealed the presence of twenty six molecules including phenolic compounds and saponins. ZARE significantly improved the heart injury markers (LDH, ALT, CK, CK-MB and cTn-I), lipid peroxidation (MDA), protein oxidation (PC), antioxidant capacity (SOD, CAT, and GPx), and DNA structure, which were altered by DLM exposure. Moreover, ZARE cotreatment reduced the expressions of NF-κB, decreased plasmatic pro-inflammatory cytokines concentration (TNF-α, IL-1β and IL-6), and suppressed the myocardial collagen deposition, as observed by Sirius Red and Masson's Trichrome staining. ZARE ameliorated the severity of DLM-induced myocardial injuries through improving the oxidative status and reducing profibrotic cytokines production. The ZARE actions could be mediated by downregulation of NF-κB mRNA.
Identifiants
pubmed: 31580943
pii: S0378-8741(19)31228-0
doi: 10.1016/j.jep.2019.112266
pii:
doi:
Substances chimiques
Anti-Inflammatory Agents
0
Cardiotonic Agents
0
NF-kappa B
0
Nitriles
0
Plant Extracts
0
Pyrethrins
0
decamethrin
2JTS8R821G
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
112266Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.