Rv0100, a proposed acyl carrier protein in Mycobacterium tuberculosis: expression, purification and crystallization.
Mycobacterium smegmatis
Mycobacterium tuberculosis
Rv0100
acyl carrier protein
Journal
Acta crystallographica. Section F, Structural biology communications
ISSN: 2053-230X
Titre abrégé: Acta Crystallogr F Struct Biol Commun
Pays: United States
ID NLM: 101620319
Informations de publication
Date de publication:
01 Oct 2019
01 Oct 2019
Historique:
received:
26
06
2019
accepted:
11
09
2019
entrez:
5
10
2019
pubmed:
5
10
2019
medline:
15
2
2020
Statut:
ppublish
Résumé
Acyl carrier proteins (ACPs) are important components in fatty-acid biosynthesis in prokaryotes. Rv0100 is predicted to be an essential ACP in Mycobacterium tuberculosis, the pathogen that is the causative agent of tuberculosis, and therefore has the potential to be a novel antituberculosis drug target. Here, the successful cloning and purification of Rv0100 using Mycobacterium smegmatis as a host is reported. Crystals of the purified protein were obtained that diffracted to a resolution of 1.9 Å. Overall, this work lays the foundation for the future pursuit of drug discovery and development against this potentially novel drug target.
Identifiants
pubmed: 31584013
pii: S2053230X19012652
doi: 10.1107/S2053230X19012652
pmc: PMC6777135
doi:
Substances chimiques
Acyl Carrier Protein
0
Bacterial Proteins
0
Recombinant Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
646-651Subventions
Organisme : NLM NIH HHS
ID : DP1 LM011509
Pays : United States
Organisme : NIH HHS
ID : DP1 OD006779
Pays : United States
Organisme : NIH HHS
ID : S10 OD024973
Pays : United States
Organisme : American Lung Association
ID : RG-82534-N
Commentaires et corrections
Type : ErratumIn
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