High-resolution melt curve analysis: A real-time based multipurpose approach for diagnosis and epidemiological investigations of parasitic infections.
HRM real-time PCR
Molecular diagnosis
Multipurpose method
Parasitic infection
Systematic review
Journal
Comparative immunology, microbiology and infectious diseases
ISSN: 1878-1667
Titre abrégé: Comp Immunol Microbiol Infect Dis
Pays: England
ID NLM: 7808924
Informations de publication
Date de publication:
Dec 2019
Dec 2019
Historique:
received:
19
06
2019
revised:
23
09
2019
accepted:
24
09
2019
pubmed:
8
10
2019
medline:
24
3
2020
entrez:
8
10
2019
Statut:
ppublish
Résumé
Real-time PCR coupled with high resolution melting curve analysis is a practical technique that could be employed in multipurpose studies. During the recent decade, this technique has been practiced for different targets, worldwide. In the current study three major database centers consisted of PubMed, Scopus and Web of Science were searched until Aug 2019 for applications of HRM real-time PCR in parasitology studies using terms: "Parasite" AND "HRM real-time PCR" OR "High Resolution Melting curve analysis" OR "Real-time PCR", "Protozoan parasites" AND "HRM real-time PCR" OR "High Resolution Melting curve analysis" OR "Real-time PCR", "Helminth" AND "HRM real-time PCR" OR "High Resolution Melting curve analysis" OR "Real-time PCR". Totally, 83 papers met our criteria and were included in our study. This method was more frequently used for protozoan parasites (52/83; 62.65%), while lower (31/83; 37.35%) studies were incorporated on helminths parasites. Furthermore, Plasmodium spp., and Leishmania spp., were the most prevalent protozoan parasites, and Taenia spp., and filers were the most frequent helminths that were studied by HRM real-time PCR. HRM real-time PCR is a sensitive, flexible and cost-effective method that could be used for multipurpose studies.
Sections du résumé
BACKGROUND
BACKGROUND
Real-time PCR coupled with high resolution melting curve analysis is a practical technique that could be employed in multipurpose studies. During the recent decade, this technique has been practiced for different targets, worldwide.
METHODS
METHODS
In the current study three major database centers consisted of PubMed, Scopus and Web of Science were searched until Aug 2019 for applications of HRM real-time PCR in parasitology studies using terms: "Parasite" AND "HRM real-time PCR" OR "High Resolution Melting curve analysis" OR "Real-time PCR", "Protozoan parasites" AND "HRM real-time PCR" OR "High Resolution Melting curve analysis" OR "Real-time PCR", "Helminth" AND "HRM real-time PCR" OR "High Resolution Melting curve analysis" OR "Real-time PCR".
RESULTS
RESULTS
Totally, 83 papers met our criteria and were included in our study. This method was more frequently used for protozoan parasites (52/83; 62.65%), while lower (31/83; 37.35%) studies were incorporated on helminths parasites. Furthermore, Plasmodium spp., and Leishmania spp., were the most prevalent protozoan parasites, and Taenia spp., and filers were the most frequent helminths that were studied by HRM real-time PCR.
CONCLUSION
CONCLUSIONS
HRM real-time PCR is a sensitive, flexible and cost-effective method that could be used for multipurpose studies.
Identifiants
pubmed: 31590033
pii: S0147-9571(19)30161-4
doi: 10.1016/j.cimid.2019.101364
pii:
doi:
Substances chimiques
DNA, Protozoan
0
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
101364Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.