Analysis of the colonic mucosa associated microbiota (MAM) using brushing samples during colonic endoscopic procedures.
16S rRNA sequencing
brushing samples
colonic mucus
mucosa associated microbiota (MAM)
taxonomic composition
Journal
Journal of clinical biochemistry and nutrition
ISSN: 0912-0009
Titre abrégé: J Clin Biochem Nutr
Pays: Japan
ID NLM: 8700907
Informations de publication
Date de publication:
Sep 2019
Sep 2019
Historique:
received:
10
01
2019
accepted:
23
04
2019
entrez:
9
10
2019
pubmed:
9
10
2019
medline:
9
10
2019
Statut:
ppublish
Résumé
The mucosa-associated microbiota is an important component in human microbiota. The aim was to investigate mucosa-associated microbiota using brush samples during endoscopic procedures and compare with fecal microbiota. Seven patients who were planning to undergo a routine colonoscopy were recruited. Mucosal brushing samples were taken from 3 sites (terminal ileum, ascending and sigmoid colon), and a fecal sample was taken on the morning of colonoscopy. The samples were immediately placed in microcentrifuge tubes containing DNA stabilization reagent and analyzed using the next generation sequencer. The individual differences in microbiota were more evident than the differences of the sampling sites. Actinobacteria was more abundant and Bacteroidetes was less in the brush samples than those in the fecal samples. Taxonomic composition at the genus level and the proportion of genes responsible for some functions in the brushing samples tended to be different from those in the fecal samples.
Identifiants
pubmed: 31592051
doi: 10.3164/jcbn.19-3
pii: DN/JST.JSTAGE/jcbn/19-3
pmc: PMC6769413
doi:
Types de publication
Journal Article
Langues
eng
Pagination
132-137Informations de copyright
Copyright © 2019 JCBNCopyright © 2019 JCBN.
Déclaration de conflit d'intérêts
The authors have no conflict of interest to declare in this study. Co-authors, YK, SH, KO and MT belong to the Miyarisan Pharmaceutical Co., Ltd.
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