Astilbin prevents bone loss in ovariectomized mice through the inhibition of RANKL-induced osteoclastogenesis.
Animals
Bone Resorption
/ prevention & control
Cells, Cultured
Drugs, Chinese Herbal
/ pharmacology
Female
Flavonols
/ pharmacology
Gene Expression
/ drug effects
Humans
Integrin beta3
/ genetics
Mice
Mice, Inbred C57BL
NFATC Transcription Factors
/ metabolism
Osteoclasts
/ drug effects
Osteogenesis
/ drug effects
Ovariectomy
Phytotherapy
/ methods
Proto-Oncogene Proteins c-fos
/ metabolism
RANK Ligand
/ pharmacology
RAW 264.7 Cells
Tartrate-Resistant Acid Phosphatase
/ genetics
Astilbin
RANKL
bone resorption
osteoclast
osteoclastogenesis
osteoporosis
Journal
Journal of cellular and molecular medicine
ISSN: 1582-4934
Titre abrégé: J Cell Mol Med
Pays: England
ID NLM: 101083777
Informations de publication
Date de publication:
12 2019
12 2019
Historique:
received:
29
01
2019
revised:
27
07
2019
accepted:
07
08
2019
pubmed:
12
10
2019
medline:
23
9
2020
entrez:
12
10
2019
Statut:
ppublish
Résumé
Osteoporosis is the most common osteolytic disease characterized by excessive osteoclast formation and resultant bone loss, which afflicts millions of patients around the world. Astilbin, a traditional herb, is known to have anti-inflammatory, antioxidant and antihepatic properties, but its role in osteoporosis treatment has not yet been confirmed. In our study, astilbin was found to have an inhibitory effect on the RANKL-induced formation and function of OCs in a dose-dependent manner without cytotoxicity. These effects were attributed to its ability to suppress the activity of two transcription factors (NFATc1 and c-Fos) indispensable for osteoclast formation, followed by inhibition of the expression of bone resorption-related genes and proteins (Acp5/TRAcP, CTSK, V-ATPase-d2 and integrin β3). Furthermore, we examined the underlying mechanisms and found that astilbin repressed osteoclastogenesis by blocking Ca
Identifiants
pubmed: 31603626
doi: 10.1111/jcmm.14713
pmc: PMC6850941
doi:
Substances chimiques
Drugs, Chinese Herbal
0
Flavonols
0
Integrin beta3
0
NFATC Transcription Factors
0
Proto-Oncogene Proteins c-fos
0
RANK Ligand
0
astilbin
29838-67-3
Acp5 protein, mouse
EC 3.1.3.2
Tartrate-Resistant Acid Phosphatase
EC 3.1.3.2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
8355-8368Informations de copyright
© 2019 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.
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