Active Fluctuations of the Nuclear Envelope Shape the Transcriptional Dynamics in Oocytes.
F-actin
bio-physics
mechano-transduction
mouse oocyte
nuclear dynamics
Journal
Developmental cell
ISSN: 1878-1551
Titre abrégé: Dev Cell
Pays: United States
ID NLM: 101120028
Informations de publication
Date de publication:
21 10 2019
21 10 2019
Historique:
received:
23
01
2019
revised:
14
06
2019
accepted:
13
09
2019
pubmed:
15
10
2019
medline:
28
5
2020
entrez:
15
10
2019
Statut:
ppublish
Résumé
Nucleus position in cells can act as a developmental cue. Mammalian oocytes position their nucleus centrally using an F-actin-mediated pressure gradient. The biological significance of nucleus centering in mammalian oocytes being unknown, we sought to assess the F-actin pressure gradient effect on the nucleus. We addressed this using a dedicated computational 3D imaging approach, biophysical analyses, and a nucleus repositioning assay in mouse oocytes mutant for cytoplasmic F-actin. We found that the cytoplasmic activity, in charge of nucleus centering, shaped the nucleus while promoting nuclear envelope fluctuations and chromatin motion. Off-centered nuclei in F-actin mutant oocytes were misshaped with immobile chromatin and modulated gene expression. Restoration of F-actin in mutant oocytes rescued nucleus architecture fully and gene expression partially. Thus, the F-actin-mediated pressure gradient also modulates nucleus dynamics in oocytes. Moreover, this study supports a mechano-transduction model whereby cytoplasmic microfilaments could modulate oocyte transcriptome, essential for subsequent embryo development.
Identifiants
pubmed: 31607652
pii: S1534-5807(19)30739-7
doi: 10.1016/j.devcel.2019.09.010
pii:
doi:
Substances chimiques
Actins
0
Chromatin
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
145-157.e10Commentaires et corrections
Type : CommentIn
Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.