Exploiting the Zonulin Mouse Model to Establish the Role of Primary Impaired Gut Barrier Function on Microbiota Composition and Immune Profiles.

Animals Disease Models, Animal Female Gastrointestinal Microbiome / immunology Haptoglobins / immunology Inflammation / immunology Interleukin-17 / immunology Intestinal Mucosa / immunology Male Mice Mice, Inbred C57BL Mice, Transgenic Microbiota / immunology Permeability Protein Precursors / immunology Tight Junctions / immunology
chronic inflammatory diseases dysbiosis gut permeability immunity microbial products trafficking microbiota tight-junctions zonulin transgenic mouse

Journal

Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960

Informations de publication

Date de publication:
2019
Historique:
received: 20 03 2019
accepted: 03 09 2019
entrez: 15 10 2019
pubmed: 15 10 2019
medline: 21 10 2020
Statut: epublish

Résumé

The balanced interplay between epithelial barrier, immune system, and microbiota maintains gut homeostasis, while disruption of this interplay may lead to inflammation. Paracellular permeability is governed by intercellular tight-junctions (TJs). Zonulin is, to date, the only known physiological regulator of intestinal TJs. We used a zonulin transgenic mouse (Ztm) model characterized by increased small intestinal permeability to elucidate the role of a primary impaired gut barrier on microbiome composition and/or immune profile. Ztm exhibit an altered gene expression profile of TJs in the gut compared to wild-type mice (WT): Claudin-15, Claudin-5, Jam-3, and Myosin-1C are decreased in the male duodenum whereas Claudin-15, Claudin-7, and ZO-2 are reduced in the female colon. These results are compatible with loss of gut barrier function and are paralleled by an altered microbiota composition with reduced abundance of the genus

Identifiants

DOI: 10.3389/fimmu.2019.02233 PMID: 31608059 PMC: PMC6761304
pubmed: 31608059
doi: 10.3389/fimmu.2019.02233
pmc: PMC6761304
doi:

Substances chimiques

Haptoglobins 0
Interleukin-17 0
Protein Precursors 0
zonulin 0

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

2233

Subventions

Organisme : NIDDK NIH HHS
ID : P30 DK040561
Pays : United States
Organisme : NIAID NIH HHS
ID : R21 AI139735
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK048373
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK104344
Pays : United States
Organisme : NIAID NIH HHS
ID : K22 AI116661
Pays : United States

Informations de copyright

Copyright © 2019 Miranda-Ribera, Ennamorati, Serena, Cetinbas, Lan, Sadreyev, Jain, Fasano and Fiorentino.

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Auteurs

Alba Miranda-Ribera (A)

Department of Pediatric Gastroenterology and Nutrition, Mucosal Immunology and Biology Research Center, Massachusetts General Hospital, Boston, MA, United States.
Department of Pediatrics, Harvard Medical School, Harvard University, Boston, MA, United States.

Maria Ennamorati (M)

Department of Pediatric Gastroenterology and Nutrition, Mucosal Immunology and Biology Research Center, Massachusetts General Hospital, Boston, MA, United States.

Gloria Serena (G)

Department of Pediatric Gastroenterology and Nutrition, Mucosal Immunology and Biology Research Center, Massachusetts General Hospital, Boston, MA, United States.
Department of Pediatrics, Harvard Medical School, Harvard University, Boston, MA, United States.

Murat Cetinbas (M)

Department of Molecular Biology, Massachusetts General Hospital, Boston, MA, United States.
Department of Genetics, Harvard Medical School, Boston, MA, United States.

Jinggang Lan (J)

Department of Pediatric Gastroenterology and Nutrition, Mucosal Immunology and Biology Research Center, Massachusetts General Hospital, Boston, MA, United States.

Ruslan I Sadreyev (RI)

Department of Molecular Biology, Massachusetts General Hospital, Boston, MA, United States.
Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, United States.

Nitya Jain (N)

Department of Pediatric Gastroenterology and Nutrition, Mucosal Immunology and Biology Research Center, Massachusetts General Hospital, Boston, MA, United States.
Department of Pediatrics, Harvard Medical School, Harvard University, Boston, MA, United States.

Alessio Fasano (A)

Department of Pediatric Gastroenterology and Nutrition, Mucosal Immunology and Biology Research Center, Massachusetts General Hospital, Boston, MA, United States.
Department of Pediatrics, Harvard Medical School, Harvard University, Boston, MA, United States.

Maria Fiorentino (M)

Department of Pediatric Gastroenterology and Nutrition, Mucosal Immunology and Biology Research Center, Massachusetts General Hospital, Boston, MA, United States.
Department of Pediatrics, Harvard Medical School, Harvard University, Boston, MA, United States.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Exploiting the Zonulin Mouse Model to...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles biologiques › Modèles animaux de maladie humaine › Exploiting the Zonulin Mouse Model to...
questionsmedicales.fr › Environnement et santé publique › Environnement › Écosystème › Biodiversité › Biote › Microbiote › Microbiome gastro-intestinal › Exploiting the Zonulin Mouse Model to...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Glycoprotéines › Mucoprotéines › Haptoglobines › Exploiting the Zonulin Mouse Model to...
questionsmedicales.fr › États, signes et symptômes pathologiques › Processus pathologiques › Inflammation › Exploiting the Zonulin Mouse Model to...
questionsmedicales.fr › Facteurs biologiques › Protéines et peptides de signalisation intercellulaire › Cytokines › Interleukines › Interleukine-17 › Exploiting the Zonulin Mouse Model to...
questionsmedicales.fr › Tissus › Membranes › Muqueuse › Muqueuse intestinale › Exploiting the Zonulin Mouse Model to...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Exploiting the Zonulin Mouse Model to...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Lignées consanguines de souris › Souris de lignée C57BL › Exploiting the Zonulin Mouse Model to...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Souris transgéniques › Exploiting the Zonulin Mouse Model to...
questionsmedicales.fr › Environnement et santé publique › Environnement › Écosystème › Biodiversité › Biote › Microbiote › Exploiting the Zonulin Mouse Model to...
questionsmedicales.fr › Phénomènes chimiques › Perméabilité › Exploiting the Zonulin Mouse Model to...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Précurseurs de protéines › Exploiting the Zonulin Mouse Model to...
questionsmedicales.fr › Cellules › Structures cellulaires › Membrane cellulaire › Structures de la membrane cellulaire › Jonctions intercellulaires › Jonctions serrées › Exploiting the Zonulin Mouse Model to...