Impact of spheroid culture on molecular and functional characteristics of bladder cancer cell lines.
bladder cancer
cell culture
cell line
chemosensitivity
molecular subtype
multicellular spheroid
Journal
Oncology letters
ISSN: 1792-1074
Titre abrégé: Oncol Lett
Pays: Greece
ID NLM: 101531236
Informations de publication
Date de publication:
Nov 2019
Nov 2019
Historique:
received:
12
03
2019
accepted:
12
06
2019
entrez:
16
10
2019
pubmed:
16
10
2019
medline:
16
10
2019
Statut:
ppublish
Résumé
The three-dimensional cell culture system is an increasingly important technique for discovering new biological aspects of cancer cells. In the present study it was demonstrated that bladder cancer cell lines, RT4 and 5637, spontaneously formed round multicellular spheroids (MCSs) in suspension by the aggregation method. MCSs consisted of cells differentially expressing luminal/basal markers. Western blotting showed that PPARγ and forkhead box A1 (FOXA1)of luminal markers were expressed to a lesser extent in MCSs than in parental cells grown in two-dimensional (2D) adherent culture. Cells in MCSs in suspension proliferated less efficiently, and were more resistant to cisplatin (CDDP) and gemcitabine than parental cells grown in 2D culture. Culturing cell lines as MCSs in suspension is a notable platform to decipher alternative biological aspects of bladder cancer cells, which could not be unraveled by the conventional 2D adherent culture.
Identifiants
pubmed: 31612003
doi: 10.3892/ol.2019.10786
pii: OL-0-0-10786
pmc: PMC6781760
doi:
Types de publication
Journal Article
Langues
eng
Pagination
4923-4929Informations de copyright
Copyright © 2019, Spandidos Publications.
Références
Oncotarget. 2018 Jan 24;9(13):11060-11070
pubmed: 29541396
Cell. 2018 Apr 5;173(2):515-528.e17
pubmed: 29625057
Oncol Rep. 2016 Apr;35(4):2431-40
pubmed: 26797047
Oncol Rep. 2015 Apr;33(4):1837-43
pubmed: 25634491
Cell Prolif. 2009 Apr;42(2):219-28
pubmed: 19222485
Eur Urol. 2018 Dec;74(6):741-753
pubmed: 30033047
J Cell Sci. 2017 Jan 1;130(1):203-218
pubmed: 27663511
PLoS One. 2014 Mar 17;9(3):e92248
pubmed: 24638075
Neoplasia. 2015 Jul;17(7):574-85
pubmed: 26297435
Cancer Cell. 2014 Feb 10;25(2):152-65
pubmed: 24525232
J Cell Biol. 1994 Feb;124(4):619-26
pubmed: 8106557
Oncol Lett. 2015 Sep;10(3):1281-1286
pubmed: 26622664
Cell. 2007 Aug 24;130(4):601-10
pubmed: 17719539
PLoS One. 2013;8(3):e59689
pubmed: 23555746
PLoS One. 2017 Aug 9;12(8):e0182930
pubmed: 28793334
Urol Oncol. 2018 Apr;36(4):160.e23-160.e32
pubmed: 29288005
Nat Rev Cancer. 2010 Apr;10(4):241-53
pubmed: 20300105
Am J Pathol. 2016 Apr;186(4):899-911
pubmed: 26878211
Int J Cancer. 2010 May 15;126(10):2448-56
pubmed: 19795452
PLoS One. 2010 May 03;5(5):e10431
pubmed: 20454659
Cell. 2018 Aug 9;174(4):1033
pubmed: 30096301
Lab Invest. 2013 May;93(5):528-42
pubmed: 23459371
Sci Rep. 2016 Dec 07;6:38531
pubmed: 27924948
EMBO Rep. 2014 Dec;15(12):1243-53
pubmed: 25381661