Screening and identification of key biomarkers in lung squamous cell carcinoma by bioinformatics analysis.
Gene Ontology enrichment analysis
Kyoto Encyclopedia of Genes and Genomes analysis
differentially expressed genes
lung squamous cell carcinoma
microarray
protein-protein interaction
Journal
Oncology letters
ISSN: 1792-1074
Titre abrégé: Oncol Lett
Pays: Greece
ID NLM: 101531236
Informations de publication
Date de publication:
Nov 2019
Nov 2019
Historique:
received:
26
04
2019
accepted:
22
08
2019
entrez:
16
10
2019
pubmed:
16
10
2019
medline:
16
10
2019
Statut:
ppublish
Résumé
The high mortality rate of lung squamous cell carcinoma (LUSC) is in part due to the lack of early detection of its biomarkers. The identification of key molecules involved in LUSC is therefore required to improve clinical diagnosis and treatment outcomes. The present study used the microarray datasets GSE31552, GSE6044 and GSE12428 from the Gene Expression Omnibus database to identify differentially expressed genes (DEGs). Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) enrichment analyses were conducted to construct the protein-protein interaction network of DEGs and hub genes module using STRING and Cytoscape. The 67 DEGs identified consisted of 42 upregulated genes and 25 downregulated genes. The pathways predicted by KEGG and GO enrichment analyses of DEGs mainly included cell cycle, cell proliferation, glycolysis or gluconeogenesis, and tetrahydrofolate metabolic process. Further analysis of the University of California Santa Cruz and ONCOMINE databases identified 17 hub genes. Overall, the present study demonstrated hub genes that were closely associated with clinical tissue samples of LUSC, and identified TYMS, CCNB2 and RFC4 as potential novel biomarkers of LUSC. The findings of the present study contribute to an improved understanding of the molecular mechanisms of carcinogenesis and progression of LUSC, and assist with the identification of potential diagnostic and therapeutic targets of LUSC.
Identifiants
pubmed: 31612029
doi: 10.3892/ol.2019.10873
pii: OL-0-0-10873
pmc: PMC6781567
doi:
Types de publication
Journal Article
Langues
eng
Pagination
5185-5196Informations de copyright
Copyright: © Man et al.
Références
Bioinformatics. 2011 Feb 1;27(3):431-2
pubmed: 21149340
Nat Cell Biol. 2009 Nov;11(11):1363-9
pubmed: 19820702
Clin Cancer Res. 2019 Jul 15;25(14):4567-4579
pubmed: 30979744
Cancer Epidemiol Biomarkers Prev. 2002 Dec;11(12):1611-21
pubmed: 12496052
Clin Cancer Res. 2015 Dec 15;21(24):5532-42
pubmed: 26156395
J Korean Med Sci. 2003 Apr;18(2):196-203
pubmed: 12692416
J Thorac Oncol. 2015 May;10(5):768-777
pubmed: 25738220
Oncotarget. 2017 Aug 12;8(45):78838-78850
pubmed: 29108269
Cell. 2011 Mar 4;144(5):646-74
pubmed: 21376230
Nucleic Acids Res. 2015 Jan;43(Database issue):D447-52
pubmed: 25352553
Ann Surg Oncol. 2018 Mar;25(3):801-807
pubmed: 29218429
Cell Death Dis. 2018 Feb 7;9(2):193
pubmed: 29416000
Aging (Albany NY). 2019 Mar 10;11(5):1333-1341
pubmed: 30853662
Thorac Cancer. 2017 Nov;8(6):672-681
pubmed: 28949095
Mol Biol (Mosk). 2011 Mar-Apr;45(2):307-15
pubmed: 21634118
Anticancer Res. 2018 Nov;38(11):6281-6290
pubmed: 30396948
Mol Cancer. 2018 May 26;17(1):93
pubmed: 29803224
Mol Biol (Mosk). 2008 Jul-Aug;42(4):652-61
pubmed: 18856066
Urol Oncol. 2016 May;34(5):237.e1-10
pubmed: 26706119
Anticancer Res. 2009 Dec;29(12):4909-18
pubmed: 20044597
Int J Cancer. 2011 Jun 15;128(12):2881-91
pubmed: 21165954
Bioinformatics. 2005 Dec 1;21(23):4205-8
pubmed: 16188928
Tumour Biol. 2016 Apr;37(4):4305-12
pubmed: 26493997
BMC Res Notes. 2012 Dec 06;5:676
pubmed: 23217244
J Pathol. 2009 Jun;218(2):182-91
pubmed: 19334046
Nat Genet. 2000 May;25(1):25-9
pubmed: 10802651
Nucleic Acids Res. 2007 Jul;35(Web Server issue):W169-75
pubmed: 17576678
Mol Clin Oncol. 2015 May;3(3):459-463
pubmed: 26137251
J Transl Med. 2014 Nov 19;12:320
pubmed: 25407051
J Thorac Dis. 2017 Jul;9(7):2054-2060
pubmed: 28840006
PLoS One. 2010 Apr 22;5(4):e10312
pubmed: 20421987
Clin Cancer Res. 2014 Nov 15;20(22):5777-86
pubmed: 25189482
Clin Cancer Res. 2018 Dec 1;24(23):5990-6000
pubmed: 30093452
Anticancer Res. 2013 May;33(5):1935-40
pubmed: 23645741
Nucleic Acids Res. 2017 Jan 4;45(D1):D353-D361
pubmed: 27899662
Biotechnol Annu Rev. 2008;14:63-108
pubmed: 18606360
Proteomics. 2015 Sep;15(17):3087-100
pubmed: 25944651
Tumour Biol. 2014 May;35(5):4257-65
pubmed: 24375198
J Thorac Oncol. 2012 May;7(5):924-33
pubmed: 22722794
Nat Commun. 2018 Aug 20;9(1):3327
pubmed: 30127402
Cancer Discov. 2012 May;2(5):401-4
pubmed: 22588877
Am J Pathol. 2014 Nov;184(11):2868-84
pubmed: 25128906
Clin Biochem. 2013 Oct;46(15):1478-83
pubmed: 23810585
Front Oncol. 2013 Aug 05;3:195
pubmed: 23936763
J Cell Biol. 2002 Jun 24;157(7):1175-86
pubmed: 12082078
PLoS One. 2018 Feb 2;13(2):e0192316
pubmed: 29394274
Br J Cancer. 2017 Jul 25;117(3):409-420
pubmed: 28641312
F1000Res. 2014 Jul 01;3:139
pubmed: 26594322
Am J Pathol. 1994 Feb;144(2):296-302
pubmed: 7906095
J Cancer. 2017 Oct 9;8(18):3641-3647
pubmed: 29151950
Int J Mol Sci. 2017 Mar 29;18(4):
pubmed: 28353639
J Thorac Oncol. 2008 Aug;3(8):819-31
pubmed: 18670299
Int J Biol Markers. 2010 Nov 23;25(4):null
pubmed: 21110300
Clin Breast Cancer. 2018 Jun;18(3):e301-e304
pubmed: 28899623
J Transl Med. 2008 Nov 07;6:69
pubmed: 18992152
Clin Cancer Res. 2004 Sep 1;10(17):5828-36
pubmed: 15355913
Clin Cancer Res. 2013 Dec 1;19(23):6556-65
pubmed: 24097870
Br J Pharmacol. 2017 Aug;174(15):2427-2443
pubmed: 28444744
Oncotarget. 2017 Feb 14;8(7):10966-10979
pubmed: 28030798
Clin Cancer Res. 2006 Feb 15;12(4):1121-7
pubmed: 16489064
Sci Signal. 2013 Apr 02;6(269):pl1
pubmed: 23550210
Nature. 2012 Sep 27;489(7417):519-25
pubmed: 22960745
Lung Cancer. 2008 Mar;59(3):377-84
pubmed: 17905465
BMC Bioinformatics. 2003 Jan 13;4:2
pubmed: 12525261
BMC Bioinformatics. 2010 Sep 27;11:483
pubmed: 20875095
Transl Lung Cancer Res. 2015 Feb;4(1):36-54
pubmed: 25806345
Expert Opin Ther Targets. 2018 Apr;22(4):295-298
pubmed: 29517391
Proc Natl Acad Sci U S A. 2001 Nov 20;98(24):13784-9
pubmed: 11707590
CA Cancer J Clin. 2017 Jan;67(1):7-30
pubmed: 28055103
Cancer Cell Int. 2018 Mar 9;18:36
pubmed: 29556139
Blood. 2007 Jun 1;109(11):4995-5001
pubmed: 17303695
Neoplasia. 2004 Jan-Feb;6(1):1-6
pubmed: 15068665
Eur Urol. 2011 May;59(5):841-8
pubmed: 21330047
Cancer Res. 2005 Jan 15;65(2):613-21
pubmed: 15695406
FASEB J. 2015 Nov;29(11):4670-81
pubmed: 26207029
Tumour Biol. 2016 May;37(5):6999
pubmed: 26825978