Development of Aptamer-Based TID Assays Using Thermophoresis and Microarrays.
Aptamer
biosensor
microarray
protein detection
targetinduced dissociation (TID)
thermophoresis
vascular endothelial growth factor (VEGF)
Journal
Biosensors
ISSN: 2079-6374
Titre abrégé: Biosensors (Basel)
Pays: Switzerland
ID NLM: 101609191
Informations de publication
Date de publication:
14 Oct 2019
14 Oct 2019
Historique:
received:
12
09
2019
revised:
01
10
2019
accepted:
09
10
2019
entrez:
17
10
2019
pubmed:
17
10
2019
medline:
20
3
2020
Statut:
epublish
Résumé
Aptamers are single-stranded oligonucleotides which can be used as alternative recognition elements for protein detection, because aptamers bind their targets with a high affinity similar to antibodies. Due to the targetinduced conformational changes of aptamers, these oligonucleotides can be applied in various biosensing platforms. In this work, aptamers directed against the vascular endothelial growth factor (VEGF) were used as a model system. VEGF plays a key role in physiological angiogenesis and vasculogenesis. Furthermore, VEGF is involved in the development and growth of cancer and other diseases like agerelated macular degeneration, rheumatoid arthritis, diabetes mellitus, and neurodegenerative disorders. Detecting the protein biomarker VEGF is therefore of great importance for medical research and diagnostics. In this research, VEGFbinding aptamers were investigated for the systematic development of a targetinduced dissociation (TID) assay utilizing thermophoresis and microarrays. The established aptamer-microarray allowed for the detection of 0.1 nM of VEGF. Furthermore, the systematic development of the TID method using the VEGF model protein could help to develop further TID assays for the detection of various protein biomarkers.
Identifiants
pubmed: 31615077
pii: bios9040124
doi: 10.3390/bios9040124
pmc: PMC6955894
pii:
doi:
Substances chimiques
Aptamers, Nucleotide
0
Vascular Endothelial Growth Factor A
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Max Buchner Research Foundation
ID : 3604
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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