Next-Generation Sequencing for the Diagnosis of Challenging Culture-Negative Endocarditis.
Cardiobacterium hominis
clinical metagenomics
culture-negative endocarditis
diagnosis
next-generation sequencing
Journal
Frontiers in medicine
ISSN: 2296-858X
Titre abrégé: Front Med (Lausanne)
Pays: Switzerland
ID NLM: 101648047
Informations de publication
Date de publication:
2019
2019
Historique:
received:
13
06
2019
accepted:
29
08
2019
entrez:
17
10
2019
pubmed:
17
10
2019
medline:
17
10
2019
Statut:
epublish
Résumé
Diagnosis of culture-negative infective endocarditis usually implies indirect pathogen identification by serologic or molecular techniques. Clinical metagenomics, relying on next-generation sequencing (NGS) is an emerging approach that allows pathogen identification in challenging situations, as evidenced by a clinical case. We sequenced the DNA extracted from the surgically-removed frozen valve tissue from a patient with suspected infective endocarditis with negative blood and valve cultures. Mapping of the sequence reads against reference genomic sequences, a 16S rRNA gene database and clade-specific marker genes suggested an infection caused by
Identifiants
pubmed: 31616669
doi: 10.3389/fmed.2019.00203
pmc: PMC6763761
doi:
Types de publication
Case Reports
Langues
eng
Pagination
203Informations de copyright
Copyright © 2019 Kolb, Lazarevic, Emonet, Calmy, Girard, Gaïa, Charretier, Cherkaoui, Keller, Huber and Schrenzel.
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