Cold Induced Depot-Specific Browning in Ferret Aortic Perivascular Adipose Tissue.
adipose tissue
browning
cardiovascular disease
cold exposure
inflammation
thermogenesis
Journal
Frontiers in physiology
ISSN: 1664-042X
Titre abrégé: Front Physiol
Pays: Switzerland
ID NLM: 101549006
Informations de publication
Date de publication:
2019
2019
Historique:
received:
11
11
2018
accepted:
29
08
2019
entrez:
18
10
2019
pubmed:
18
10
2019
medline:
18
10
2019
Statut:
epublish
Résumé
Brown adipose tissue is responsible for facultative thermogenesis to produce heat and increase energy expenditure in response to proper stimuli, e.g., cold. Acquisition of brown-like features (browning) in perivascular white adipose tissue (PVAT) may protect against obesity/cardiovascular disease. Most browning studies are performed in rodents, but translation to humans would benefit from a closer animal model. Therefore, we studied the browning response of ferret thoracic aortic PVAT (tPVAT) to cold. We performed global transcriptome analysis of tPVAT of 3-month-old ferrets acclimatized 1 week to 22 or 4°C, and compared the results with those of inguinal subcutaneous adipose tissue. Immunohistochemistry was used to visualize browning. Transcriptome data revealed a stronger cold exposure response of tPVAT, including increased expression of key brown/brite markers, compared to subcutaneous fat. This translated into a clear white-to-brown remodeling of tPVAT, with the appearance of multilocular highly UCP1-stained adipocytes. The pathway most affected by cold exposure in tPVAT was immune response, characterized by down-regulation of immune-related genes, with cardio protective implications. On the other hand, subcutaneous fat responded to cold by increasing energy metabolism based on increased expression of fatty acid oxidation and tricarboxylic acid cycle genes, concordant with lower inguinal adipose tissue weight in cold-exposed animals. Thus, ferret tPVAT responds to cold acclimation with a strong induction of browning and immunosuppression compared to subcutaneous fat. Our results present ferrets as an accessible translational animal model displaying functional responses relevant for obesity and cardiovascular disease prevention.
Identifiants
pubmed: 31620014
doi: 10.3389/fphys.2019.01171
pmc: PMC6759601
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1171Informations de copyright
Copyright © 2019 Reynés, van Schothorst, Keijer, Ceresi, Oliver and Palou.
Références
Circ Res. 2009 Feb 27;104(4):541-9
pubmed: 19122178
Br J Pharmacol. 2012 Feb;165(3):659-69
pubmed: 21545577
Br J Pharmacol. 2017 Oct;174(20):3496-3513
pubmed: 28063251
Int J Obes Relat Metab Disord. 2004 Dec;28 Suppl 4:S58-65
pubmed: 15592488
Biochim Biophys Acta. 2013 May;1831(5):969-85
pubmed: 23246573
Nature. 2012 Jan 11;481(7382):463-8
pubmed: 22237023
Front Physiol. 2017 Dec 19;8:1032
pubmed: 29311966
Physiol Genomics. 2003 Dec 16;16(1):99-106
pubmed: 14570982
Cell Metab. 2018 Jan 9;27(1):180-194.e6
pubmed: 29320702
Thromb Haemost. 2017 May 3;117(5):981-991
pubmed: 28251235
Prostaglandins Leukot Essent Fatty Acids. 2005 Jul;73(1):9-15
pubmed: 15936182
Int J Cardiol. 2017 Feb 1;228:265-274
pubmed: 27865196
Int J Obes (Lond). 2014 Feb;38(2):170-6
pubmed: 23736373
Anal Biochem. 2007 Apr 15;363(2):315-7
pubmed: 17320029
Differentiation. 1998 Aug;63(4):193-200
pubmed: 9745710
Int J Biochem Cell Biol. 1998 Jan;30(1):7-11
pubmed: 9597749
PLoS One. 2018 Mar 19;13(3):e0194269
pubmed: 29554135
Mol Metab. 2018 Mar;9:199-206
pubmed: 29396370
Cell. 2012 Jul 20;150(2):366-76
pubmed: 22796012
J Am Coll Nutr. 2015;34(3):244-54
pubmed: 25760239
Science. 2010 May 28;328(5982):1113-4
pubmed: 20448151
Cell Mol Life Sci. 2017 May;74(10):1765-1776
pubmed: 27866221
Diabetes Metab J. 2013 Feb;37(1):22-9
pubmed: 23441053
Bioinformatics. 2004 Dec 12;20(18):3705-6
pubmed: 15297296
Br J Nutr. 2009 Dec;102(11):1686-94
pubmed: 19631020
Physiol Rev. 2004 Jan;84(1):277-359
pubmed: 14715917
Int J Obes (Lond). 2015 Nov;39(11):1619-29
pubmed: 26063331
J Histochem Cytochem. 1981 Apr;29(4):577-80
pubmed: 6166661
Comp Biochem Physiol A Comp Physiol. 1983;74(2):225-30
pubmed: 6131765
Am J Physiol Endocrinol Metab. 2015 Jan 15;308(2):E172-83
pubmed: 25406261
Metab Syndr Relat Disord. 2017 Nov;15(9):450-457
pubmed: 28934021
Int J Obes (Lond). 2017 Feb;41(2):309-316
pubmed: 27840413
Front Microbiol. 2016 Feb 04;7:80
pubmed: 26870031
Acta Physiol Scand. 2005 Aug;184(4):285-93
pubmed: 16026420
J Biol Chem. 2010 Mar 5;285(10):7153-64
pubmed: 20028987
Nucleic Acids Res. 2001 May 1;29(9):e45
pubmed: 11328886
J Histochem Cytochem. 1993 Oct;41(10):1447-57
pubmed: 8245404
J Clin Invest. 2011 Jan;121(1):96-105
pubmed: 21123942
Am J Physiol Endocrinol Metab. 2007 Aug;293(2):E444-52
pubmed: 17473055
Front Cardiovasc Med. 2017 May 05;4:27
pubmed: 28529941
Mol Metab. 2012 Nov 08;2(1):5-9
pubmed: 24024125
Viruses. 2016 Jun 14;8(6):
pubmed: 27314379
Int J Obes (Lond). 2017 Jun;41(6):894-901
pubmed: 27916987
J Intern Med. 2018 Nov;284(5):492-504
pubmed: 29923291
Mol Cell Biol. 2009 Mar;29(6):1575-91
pubmed: 19114551
Am J Physiol Heart Circ Physiol. 2011 Oct;301(4):H1425-37
pubmed: 21765057
Integr Comp Biol. 2009 Sep;49(3):246-53
pubmed: 21665817
Comp Biochem Physiol A Mol Integr Physiol. 2009 Jun;153(2):106-12
pubmed: 19535042
J Ultrastruct Res. 1969 Feb;26(3):216-41
pubmed: 5776618
Front Endocrinol (Lausanne). 2016 Apr 13;7:30
pubmed: 27148161
J Cell Sci. 1992 Dec;103 ( Pt 4):931-42
pubmed: 1362571