Cell Type- and Sex-Dependent Transcriptome Profiles of Rat Anterior Pituitary Cells.
folliculostellate cells
hormone-producing cells
pituitary gland
rat
sexual dimorphism
single-cell RNA sequencing
transcriptome
Journal
Frontiers in endocrinology
ISSN: 1664-2392
Titre abrégé: Front Endocrinol (Lausanne)
Pays: Switzerland
ID NLM: 101555782
Informations de publication
Date de publication:
2019
2019
Historique:
received:
12
06
2019
accepted:
28
08
2019
entrez:
18
10
2019
pubmed:
18
10
2019
medline:
18
10
2019
Statut:
epublish
Résumé
Understanding the physiology and pathology of an organ composed of a variety of cell populations depends critically on genome-wide information on each cell type. Here, we report single-cell transcriptome profiling of over 6,800 freshly dispersed anterior pituitary cells from postpubertal male and female rats. Six pituitary-specific cell types were identified based on known marker genes and characterized: folliculostellate cells and hormone-producing corticotrophs, gonadotrophs, thyrotrophs, somatotrophs, and lactotrophs. Also identified were endothelial and blood cells from the pituitary capillary network. The expression of numerous developmental and neuroendocrine marker genes in both folliculostellate and hormone-producing cells supports that they have a common origin. For several genes, the validity of transcriptome analysis was confirmed by qRT-PCR and single cell immunocytochemistry. Folliculostellate cells exhibit impressive transcriptome diversity, indicating their major roles in production of endogenous ligands and detoxification enzymes, and organization of extracellular matrix. Transcriptome profiles of hormone-producing cells also indicate contributions toward those functions, while also clearly demonstrating their endocrine function. This survey highlights many novel genetic markers contributing to pituitary cell type identity, sexual dimorphism, and function, and points to relationships between hormone-producing and folliculostellate cells.
Identifiants
pubmed: 31620083
doi: 10.3389/fendo.2019.00623
pmc: PMC6760010
doi:
Types de publication
Journal Article
Langues
eng
Pagination
623Subventions
Organisme : Intramural NIH HHS
ID : Z01 DK013028
Pays : United States
Organisme : Intramural NIH HHS
ID : Z01 HD000195
Pays : United States
Informations de copyright
Copyright © 2019 Fletcher, Smiljanic, Maso Prévide, Iben, Li, Rokic, Sherman, Coon and Stojilkovic.
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