Identification of Transgene-Free CRISPR-Edited Plants of Rice, Tomato, and
Arabidopsis thaliana
CRISPR/Cas9
DsRED
Oryza sativa
Solanum lycopersicum
genome editing
Journal
Frontiers in plant science
ISSN: 1664-462X
Titre abrégé: Front Plant Sci
Pays: Switzerland
ID NLM: 101568200
Informations de publication
Date de publication:
2019
2019
Historique:
received:
10
07
2019
accepted:
23
08
2019
entrez:
18
10
2019
pubmed:
18
10
2019
medline:
18
10
2019
Statut:
epublish
Résumé
Efficient elimination of the editing machinery remains a challenge in plant biotechnology after genome editing to minimize the probability of off-target mutations, but it is also important to deliver end users with edited plants free of foreign DNA. Using the modular cloning system Golden Braid, we have included a fluorescence-dependent transgene monitoring module to the genome-editing tool box. We have tested this approach in
Identifiants
pubmed: 31620160
doi: 10.3389/fpls.2019.01150
pmc: PMC6759815
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1150Informations de copyright
Copyright © 2019 Aliaga-Franco, Zhang, Presa, Srivastava, Granell, Alabadí, Sadanandom, Blázquez and Minguet.
Références
Methods Mol Biol. 2019;1917:147-154
pubmed: 30610634
Plant Methods. 2016 Feb 01;12:10
pubmed: 26839579
Plant Methods. 2018 Aug 2;14:65
pubmed: 30083222
Bioinformatics. 2007 Nov 1;23(21):2947-8
pubmed: 17846036
Nucleic Acids Res. 2014 Dec 16;42(22):e168
pubmed: 25300484
Plant Physiol. 2013 Jul;162(3):1618-31
pubmed: 23669743
Development. 2003 Dec;130(24):6065-73
pubmed: 14597573
Plant J. 1998 Dec;16(6):735-43
pubmed: 10069079
Proc Natl Acad Sci U S A. 2018 Jun 26;115(26):6864-6869
pubmed: 29899148
Methods Mol Biol. 2015;1284:399-420
pubmed: 25757784
Plant Biotechnol J. 2003 Jul;1(4):301-9
pubmed: 17163906
Methods Mol Biol. 1998;82:259-66
pubmed: 9664431
Biotechniques. 2019 Jun;66(6):295-302
pubmed: 31039627
Nat Protoc. 2008;3(5):824-34
pubmed: 18451790
Nature. 2012 Feb 15;482(7385):331-8
pubmed: 22337052
Theor Appl Genet. 2003 Jan;106(2):231-8
pubmed: 12582848
Plant Physiol. 2016 Jul;171(3):1794-800
pubmed: 27208253
Nucleic Acids Res. 2013 Nov;41(20):e188
pubmed: 23999092
Plant Biotechnol J. 2018 Jan;16(1):272-279
pubmed: 28574629
Plant J. 2006 Sep;47(6):969-76
pubmed: 16961734
Methods Mol Biol. 2014;1116:133-51
pubmed: 24395362
Trends Genet. 2008 Nov;24(11):529-38
pubmed: 18809224
Plant Cell. 2005 Oct;17(10):2693-704
pubmed: 16169896
Sci Rep. 2018 Mar 13;8(1):4443
pubmed: 29535386
Plant J. 2010 Feb 1;61(3):519-28
pubmed: 19891705
J Mol Evol. 2005 Feb;60(2):174-82
pubmed: 15791728
Nucleic Acids Res. 1991 Mar 25;19(6):1349
pubmed: 2030957
Nat Commun. 2014 Oct 31;5:5334
pubmed: 25358957
Microbiology. 2005 Aug;151(Pt 8):2551-61
pubmed: 16079334
Science. 2012 Aug 17;337(6096):816-21
pubmed: 22745249
Plant Physiol. 2008 Jul;147(3):1034-45
pubmed: 18467465
Cell Res. 2013 Oct;23(10):1229-32
pubmed: 23958582
Plant Biotechnol J. 2017 Jun;15(6):729-739
pubmed: 27885771
J Biotechnol. 2004 Feb 5;107(3):193-232
pubmed: 14736458
Nucleic Acids Res. 2015 Jan;43(Database issue):D1036-41
pubmed: 25428362
Plant Biotechnol J. 2019 Oct;17(10):1905-1913
pubmed: 30839150
New Phytol. 2005 Jul;167(1):9-18
pubmed: 15948825
Nucleic Acids Res. 1980 Oct 10;8(19):4321-5
pubmed: 7433111
BMC Biotechnol. 2013 Apr 23;13:36
pubmed: 23617583
Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3570-5
pubmed: 25733849
Biotechnol J. 2007 Jan;2(1):83-90
pubmed: 17167767
Plant Biotechnol J. 2019 Oct;17(10):1971-1984
pubmed: 30950179