Comparison of Theiler's Murine Encephalomyelitis Virus Induced Spinal Cord and Peripheral Nerve Lesions Following Intracerebral and Intraspinal Infection.
Theiler’s murine encephalomyelitis virus-induced demyelinating disease
inflammation
intraspinal infection
peripheral nerve lesions
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
16 Oct 2019
16 Oct 2019
Historique:
received:
24
09
2019
revised:
14
10
2019
accepted:
15
10
2019
entrez:
19
10
2019
pubmed:
19
10
2019
medline:
27
2
2020
Statut:
epublish
Résumé
Hallmarks of Theiler's murine encephalomyelitis virus (TMEV)-induced demyelinating disease (TMEV-IDD) include spinal cord (SC) inflammation, demyelination and axonal damage occurring approximately 5-8 weeks after classical intracerebral (i.c.) infection. The aim of this study was to elucidate the consequences of intraspinal (i.s.) TMEV infection and a direct comparison of classical i.c. and intraspinal infection. Swiss Jim Lambert (SJL)-mice were i.s. infected with the BeAn strain of TMEV. Clinical investigations including a scoring system and rotarod analysis were performed on a regular basis. Necropsies were performed at 3, 7, 14, 28 and 63 days post infection (dpi) following i.s. and at 4, 7, 14, 28, 56, 98, 147 and 196 dpi following i.c. infection. Serial sections of formalin-fixed, paraffin-embedded SC and peripheral nerves (PN) were investigated using hematoxylin and eosin (HE) and immunohistochemistry. I.s. infected mice developed clinical signs and a deterioration of motor coordination approximately 12 weeks earlier than i.c. infected animals. SC inflammation, demyelination and axonal damage occurred approximately 6 weeks earlier in i.s. infected animals. Interestingly, i.s. infected mice developed PN lesions, characterized by vacuolation, inflammation, demyelination and axonal damage, which was not seen following i.c. infection. The i.s. infection model offers the advantage of a significantly earlier onset of clinical signs, inflammatory and demyelinating SC lesions and additionally enables the investigation of virus-mediated PN lesions.
Identifiants
pubmed: 31623261
pii: ijms20205134
doi: 10.3390/ijms20205134
pmc: PMC6834305
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Ministry of Science and Culture of Lower Saxony
ID : Niedersachsen-Research Network on Neuroinfectiology (N-RENNT)
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
Références
Virology. 2007 Mar 1;359(1):233-42
pubmed: 17028060
Muscle Nerve. 1997 Jan;20(1):72-82
pubmed: 8995586
Eur J Neurol. 2019 Jan;26(1):27-40
pubmed: 30300457
Pharmacoeconomics. 2018 Feb;36(2):189-204
pubmed: 29032493
Infect Immun. 1975 May;11(5):1147-55
pubmed: 164412
Curr Treat Options Neurol. 2018 Apr 7;20(5):12
pubmed: 29627873
Apoptosis. 2018 Feb;23(2):170-186
pubmed: 29435686
Cochrane Database Syst Rev. 2013 Dec 06;(12):CD009130
pubmed: 24310855
Neuroscience. 2002;111(2):399-411
pubmed: 11983325
Prog Neurobiol. 2013 Feb-Mar;101-102:46-64
pubmed: 23201558
Acta Neuropathol. 2017 Feb;133(2):223-244
pubmed: 27766432
J Pathol. 1988 Dec;156(4):331-9
pubmed: 3265723
J Neurovirol. 2014 Apr;20(2):107-12
pubmed: 23857332
Science. 1976 Apr 2;192(4234):62-4
pubmed: 176726
J Virol. 1987 May;61(5):1507-16
pubmed: 3033278
Biol Chem. 2014 Sep;395(9):1063-73
pubmed: 25153387
Neurology. 2007 May 29;68(22 Suppl 3):S5-12; discussion S43-54
pubmed: 17548569
Int J Mol Sci. 2019 Jan 21;20(2):null
pubmed: 30669615
Infect Immun. 1979 Oct;26(1):369-74
pubmed: 500211
J Neuroimmunol. 2000 Apr 3;104(1):79-84
pubmed: 10683517
Curr Opin Infect Dis. 2008 Jun;21(3):271-8
pubmed: 18448972
J Virol Methods. 2009 Sep;160(1-2):185-8
pubmed: 19414033
Nat Rev Neurosci. 2009 Jan;10(1):9-22
pubmed: 19096367
Nat Med. 1997 Oct;3(10):1133-6
pubmed: 9334726
Ann N Y Acad Sci. 2007 Apr;1103:152-6
pubmed: 17376832
Neuropathol Appl Neurobiol. 2008 Dec;34(6):603-20
pubmed: 18466224
J Exp Med. 1937 Apr 30;65(5):705-19
pubmed: 19870629
J Virol. 2005 Jul;79(13):8581-90
pubmed: 15956599
J Exp Med. 1949 Jan;89(1):69-92
pubmed: 18099166
Neurol Res Int. 2011;2011:383087
pubmed: 22203900
J Virol. 1999 Jul;73(7):6093-8
pubmed: 10364364
Microb Pathog. 2006 Oct-Nov;41(4-5):149-56
pubmed: 16935465
Brain Pathol. 2012 Jul;22(4):454-71
pubmed: 21988534
Vet Immunol Immunopathol. 2018 Aug;202:130-140
pubmed: 30078587
Mol Cell Neurosci. 2003 Apr;22(4):430-40
pubmed: 12727441
Brain Pathol. 2010 Sep;20(5):890-901
pubmed: 20331618
J Exp Med. 1940 Jun 30;72(1):49-67
pubmed: 19871007
Neurology. 2007 May 29;68(22 Suppl 3):S13-21; discussion S43-54
pubmed: 17548563
Clin Microbiol Rev. 2004 Jan;17(1):174-207
pubmed: 14726460
Autoimmun Rev. 2002 Oct;1(5):251-60
pubmed: 12848977
J Immunol. 1989 Apr 15;142(8):2677-82
pubmed: 2495323
Ann Neurol. 1979 Jul;6(1):25-8
pubmed: 228588
Sci Rep. 2019 Mar 14;9(1):4588
pubmed: 30872675
Cell Stem Cell. 2010 Jun 4;6(6):578-90
pubmed: 20569695
Brain Res. 2016 May 1;1638(Pt B):199-208
pubmed: 26232070
Clin Exp Neuroimmunol. 2016 Nov;7(4):330-345
pubmed: 28603559
Acta Neuropathol. 2012 Jul;124(1):127-42
pubmed: 22271152
Annu Rev Neurosci. 2008;31:247-69
pubmed: 18558855
Discov Med. 2014 Jul-Aug;18(97):79-89
pubmed: 25091490
Glia. 2014 Jun;62(6):896-913
pubmed: 24578301
Noro Psikiyatr Ars. 2018 Jul 06;56(2):115-118
pubmed: 31223243
Lab Invest. 1975 Dec;33(6):626-37
pubmed: 1202282
J Neurosci. 2014 Jul 30;34(31):10285-97
pubmed: 25080590
Brain Pathol. 2007 Apr;17(2):210-8
pubmed: 17388952
Am J Pathol. 2003 Apr;162(4):1259-69
pubmed: 12651618
Annu Rev Microbiol. 2005;59:279-98
pubmed: 16153171
Springer Semin Immunopathol. 2002;24(2):105-25
pubmed: 12503060
J Immunol. 2000 Nov 1;165(9):5304-14
pubmed: 11046065
Brain Res. 2016 May 1;1638(Pt B):116-128
pubmed: 26301825
Curr Neuropharmacol. 2010 Sep;8(3):305-15
pubmed: 21358979
Transgenic Res. 2010 Aug;19(4):715-25
pubmed: 19894134
Int J Mol Sci. 2019 Feb 25;20(4):null
pubmed: 30823515
Methods. 1996 Dec;10(3):453-61
pubmed: 8954856
Neuropathol Appl Neurobiol. 2015 Apr;41(3):347-70
pubmed: 24606160