Relationship between DNA fragmentation of equine granulosa cells and oocyte meiotic competence after in vitro maturation.
granulosa cells
mare
meiotic competence
oocyte
sperm chromatin dispersion
Journal
Reproduction in domestic animals = Zuchthygiene
ISSN: 1439-0531
Titre abrégé: Reprod Domest Anim
Pays: Germany
ID NLM: 9015668
Informations de publication
Date de publication:
Oct 2019
Oct 2019
Historique:
received:
30
04
2019
revised:
08
07
2019
accepted:
11
07
2019
entrez:
19
10
2019
pubmed:
19
10
2019
medline:
3
3
2020
Statut:
ppublish
Résumé
The acquisition of equine oocyte developmental capacity is ensured by the follicular environment, such as granulosa cells, which could reflect the meiotic development potential of immature oocytes. This study evaluated the relationship between DNA fragmentation of granulosa cells, using the chromatin dispersion test, and equine oocyte meiotic development after in vitro maturation. Granulosa cells and cumulus-oocytes complexes (n = 50) were recovered from slaughterhouse-derived ovaries. Oocytes were in vitro matured, stained and evaluated under fluorescence microscopy. Maturation rates were classified into outstanding, medium and poor levels of maturation using 25th and 75th percentiles as thresholds. For DNA assessment, each sample was processed with the Ovoselect
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
78-81Subventions
Organisme : Conserjería de Conocimiento, Investigación y Universidad (Junta de Andalucía, Spain)
ID : EJI-17-AGR-275
Organisme : European Social Fund
ID : ES-F_2014-2020
Organisme : MINECO
ID : AGL2013-42726-R
Informations de copyright
© 2019 Blackwell Verlag GmbH.
Références
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