Nuclear Pores Assemble from Nucleoporin Condensates During Oogenesis.
NPC assembly
Nup358
RNA localization
Ran
local translation
microtubule-dependent transport
nuclear pore complex
nucleoporin condensation
oogenesis
Journal
Cell
ISSN: 1097-4172
Titre abrégé: Cell
Pays: United States
ID NLM: 0413066
Informations de publication
Date de publication:
17 10 2019
17 10 2019
Historique:
received:
20
11
2018
revised:
09
08
2019
accepted:
20
09
2019
entrez:
19
10
2019
pubmed:
19
10
2019
medline:
28
5
2020
Statut:
ppublish
Résumé
The molecular events that direct nuclear pore complex (NPC) assembly toward nuclear envelopes have been conceptualized in two pathways that occur during mitosis or interphase, respectively. In gametes and embryonic cells, NPCs also occur within stacked cytoplasmic membrane sheets, termed annulate lamellae (AL), which serve as NPC storage for early development. The mechanism of NPC biogenesis at cytoplasmic membranes remains unknown. Here, we show that during Drosophila oogenesis, Nucleoporins condense into different precursor granules that interact and progress into NPCs. Nup358 is a key player that condenses into NPC assembly platforms while its mRNA localizes to their surface in a translation-dependent manner. In concert, Microtubule-dependent transport, the small GTPase Ran and nuclear transport receptors regulate NPC biogenesis in oocytes. We delineate a non-canonical NPC assembly mechanism that relies on Nucleoporin condensates and occurs away from the nucleus under conditions of cell cycle arrest.
Identifiants
pubmed: 31626769
pii: S0092-8674(19)31073-6
doi: 10.1016/j.cell.2019.09.022
pmc: PMC6838685
pii:
doi:
Substances chimiques
Drosophila Proteins
0
Molecular Chaperones
0
Nuclear Pore Complex Proteins
0
RNA, Messenger
0
ran-binding protein 2
0
ran GTP-Binding Protein
EC 3.6.5.2
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
671-686.e17Subventions
Organisme : NIH HHS
ID : P40 OD018537
Pays : United States
Commentaires et corrections
Type : CommentIn
Type : CommentIn
Type : CommentIn
Informations de copyright
Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.
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