An array of multiplex PCR assays for detection of staphylococcal chromosomal cassette mec (SCCmec) types among staphylococcal isolates.

Bacterial Proteins / genetics Bacterial Typing Techniques / methods Chromosomes, Bacterial / genetics Humans Methicillin Resistance / genetics Methicillin-Resistant Staphylococcus aureus / genetics Microbial Sensitivity Tests / methods Multiplex Polymerase Chain Reaction / methods Staphylococcal Infections / microbiology

Journal

Journal of microbiological methods
ISSN: 1872-8359
Titre abrégé: J Microbiol Methods
Pays: Netherlands
ID NLM: 8306883

Informations de publication

Date de publication:
11 2019
Historique:
received: 13 06 2019
revised: 26 09 2019
accepted: 29 09 2019
pubmed: 20 10 2019
medline: 24 7 2020
entrez: 20 10 2019
Statut: ppublish

Résumé

Staphylococcal Cassette Chromosome mec (SCCmec) element plays a key role in development of resistance by carrying different resistance factors. Therefore, routine and rapid diagnostic approach is considered advantageous for the easy detection of SCCmec elements. So, here we have described the use of three sets of multiplex PCR assay, which can be used to identify SCCmec type I to type XII, unlike other known protocols. MRSA isolates of both hospital and community settings had been utilized to confirm the sensitivity of the method. All the isolates were examined for SCCmec types using multiplex PCR assay followed by sequencing of amplified products. The results confirmed the detection of SCCmec type I, type II, type III, type IV, type V, type VI, type VII, type VIII and type XII, where SCCmec type II having ST1551 and type V with ST2416 were found to be associated with multidrug resistance and were highly prevalent in the study area. This method will be useful for epidemiological assessment as it will be easier to track the resistance among staphylococci for control of infections and its management.

Identifiants

DOI: 10.1016/j.mimet.2019.105733 PMID: 31629020
pubmed: 31629020
pii: S0167-7012(19)30494-4
doi: 10.1016/j.mimet.2019.105733
pii:
doi:

Substances chimiques

Bacterial Proteins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

105733

Informations de copyright

Copyright © 2019 Elsevier B.V. All rights reserved.

Auteurs

Deepshikha Bhowmik (D)

Department of Microbiology, Assam University, Silchar, India.

Bhaskar Jyoti Das (BJ)

Department of Microbiology, Assam University, Silchar, India.

Piyush Pandey (P)

Department of Microbiology, Assam University, Silchar, India.

Shiela Chetri (S)

Department of Microbiology, Assam University, Silchar, India.

Debadatta Dhar Chanda (DD)

Department of Microbiology, Silchar Medical College and Hospital, Silchar, India.

Amitabha Bhattacharjee (A)

Department of Microbiology, Assam University, Silchar, India. Electronic address: ab0404@gmail.com.

Articles similaires

[Redispensing of expensive oral anticancer medicines: a practical application].

Lisanne N van Merendonk, Kübra Akgöl, Bastiaan Nuijen
1.00
Humans Antineoplastic Agents Administration, Oral Drug Costs Counterfeit Drugs

Smoking Cessation and Incident Cardiovascular Disease.

Jun Hwan Cho, Seung Yong Shin, Hoseob Kim et al.
1.00
Humans Male Smoking Cessation Cardiovascular Diseases Female

Evaluation of Low-Value Services Across Major Medicare Advantage Insurers and Traditional Medicare.

Ciara Duggan, Adam L Beckman, Ishani Ganguli et al.
1.00
Humans United States Aged Cross-Sectional Studies Medicare Part C

Effectiveness of Virtual Yoga for Chronic Low Back Pain: A Randomized Clinical Trial.

Hallie Tankha, Devyn Gaskins, Amanda Shallcross et al.
1.00
Humans Yoga Low Back Pain Female Male

Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › An array of multiplex PCR assays for detection...
questionsmedicales.fr › Techniques d'investigation › Techniques de laboratoire clinique › Techniques microbiologiques › Techniques bactériologiques › Techniques de typage bactérien › An array of multiplex PCR assays for detection...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Chromosomes › Chromosomes de bactérie › An array of multiplex PCR assays for detection...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › An array of multiplex PCR assays for detection...
questionsmedicales.fr › Phénomènes physiologiques › Phénomènes pharmacologiques et toxicologiques › Phénomènes pharmacologiques › Résistance aux substances › Résistance microbienne aux médicaments › Résistance bactérienne aux médicaments › Résistance aux bêta-lactamines › Résistance aux pénicillines › Résistance à la méticilline › An array of multiplex PCR assays for detection...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Cocci à Gram positif › Staphylococcaceae › Staphylococcus › Staphylococcus aureus › Staphylococcus aureus résistant à la méticilline › An array of multiplex PCR assays for detection...
questionsmedicales.fr › Techniques d'investigation › Évaluation préclinique de médicament › Tests de sensibilité microbienne › An array of multiplex PCR assays for detection...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › Réaction de polymérisation en chaine multiplex › An array of multiplex PCR assays for detection...
questionsmedicales.fr › Infection › Infections bactériennes et mycoses › Infections bactériennes › Infections bactériennes à Gram positif › Infections à staphylocoques › An array of multiplex PCR assays for detection...