Antimicrobial, anti-biofilm, and anti-proliferative activities of lipopeptide biosurfactant produced by Acinetobacter junii B6.
Acinetobacter
/ metabolism
Antimicrobial Cationic Peptides
/ biosynthesis
Bacteria
/ drug effects
Biofilms
/ drug effects
Cell Survival
/ drug effects
Dose-Response Relationship, Drug
Fungi
/ drug effects
Human Umbilical Vein Endothelial Cells
/ drug effects
Humans
Lipopeptides
/ biosynthesis
Microbial Sensitivity Tests
Staphylococcus aureus
/ drug effects
Surface-Active Agents
/ metabolism
Acinetobacter junii
Anti-biofilm
Anti-proliferative
Antimicrobial
Biosurfactant
Lipopeptide
Journal
Microbial pathogenesis
ISSN: 1096-1208
Titre abrégé: Microb Pathog
Pays: England
ID NLM: 8606191
Informations de publication
Date de publication:
Jan 2020
Jan 2020
Historique:
received:
20
02
2019
revised:
15
10
2019
accepted:
15
10
2019
pubmed:
21
10
2019
medline:
21
10
2020
entrez:
21
10
2019
Statut:
ppublish
Résumé
Lipopeptide biosurfactants (LPBs) are amphiphilic compounds produced by microorganisms exhibiting various biological activities. The main aim of the present study was to assess the in vitro antimicrobial, anti-biofilm, and cytotoxic effects of LPB produced by Acinetobacter junii (AjL). We determined AjL minimum inhibitory concentration (MIC) against both Gram-positive and Gram-negative bacteria as well as two fungal strains. Also, the anti-biofilm activity of AjL against the biofilm produced by clinically isolated bacterial strains was investigated. The AjL non-selectively showed activity against both Gram-positive and Gram-negative bacterial strains. The obtained results of the present study exhibited that the AjL in concentrations nearly below critical micelle concentration (CMC) has an effective antibacterial activity. It was found that the MIC values of AjL were lower than standard antifungal and it exhibited nearly 100% inhibition against Candida utilis. The attained results of the biofilm formation revealed that AjL disrupted the biofilm of Proteus mirabilis, Staphylococcus aureus, and Pseudomonas aeruginosa at 1250 μg/ml and 2500 μg/ml concentrations. The attained results of cytotoxic effect (determined by WST-1 assay) of the AjL revealed IC
Identifiants
pubmed: 31629797
pii: S0882-4010(19)30325-0
doi: 10.1016/j.micpath.2019.103806
pii:
doi:
Substances chimiques
Antimicrobial Cationic Peptides
0
Lipopeptides
0
Surface-Active Agents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
103806Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.