Awakening dormant glycosyltransferases in CHO cells with CRISPRa.
CHO
CRISPRa
Mgat3
St6gal1
glycosylation
Journal
Biotechnology and bioengineering
ISSN: 1097-0290
Titre abrégé: Biotechnol Bioeng
Pays: United States
ID NLM: 7502021
Informations de publication
Date de publication:
02 2020
02 2020
Historique:
received:
08
07
2019
revised:
09
10
2019
accepted:
15
10
2019
pubmed:
22
10
2019
medline:
7
2
2021
entrez:
22
10
2019
Statut:
ppublish
Résumé
Chinese hamster ovary (CHO) cells are the preferred workhorse for the biopharmaceutical industry, and CRISPR/Cas9 has proven powerful for generating targeted gene perturbations in CHO cells. Here, we expand the CRISPR engineering toolbox with CRISPR activation (CRISPRa) to increase transcription of endogenous genes. We successfully increased transcription of Mgat3 and St6gal1, and verified their activity on a functional level by subsequently detecting that the appropriate glycan structures were produced. This study demonstrates that CRISPRa can make targeted alterations of CHO cells for desired phenotypes.
Identifiants
pubmed: 31631317
doi: 10.1002/bit.27199
pmc: PMC7261405
mid: NIHMS1592607
doi:
Substances chimiques
Polysaccharides
0
Glycosyltransferases
EC 2.4.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
593-598Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM119850
Pays : United States
Organisme : Novo Nordisk Foundation
ID : NNF16OC0021638
Pays : International
Organisme : Novo Nordisk Foundation
ID : NNF10CC1016517
Pays : International
Informations de copyright
© 2019 Wiley Periodicals, Inc.
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