Atomic Force Microscopy Is a Potent Technique to Study Eosinophil Activation.
acute asthma
advanced microscopy
allergic diseases
atomic force microscopy
cell morphological changes
eosinophils
Journal
Frontiers in physiology
ISSN: 1664-042X
Titre abrégé: Front Physiol
Pays: Switzerland
ID NLM: 101549006
Informations de publication
Date de publication:
2019
2019
Historique:
received:
03
05
2019
accepted:
17
09
2019
entrez:
22
10
2019
pubmed:
22
10
2019
medline:
22
10
2019
Statut:
epublish
Résumé
Eosinophils are multifunctional cells with several functions both in healthy individuals, and those with several diseases. Increased number and morphological changes in eosinophils have been correlated with the severity of an acute asthma exacerbation. We measured eosinophils obtained from healthy controls and individuals with acute asthma using atomic force microscopy (AFM). In the control samples, cells showed more rounded morphologies with some spreading, while activated cells from symptomatic individuals were spreading, and presenting emission of multiple pseudopods. Eosinophils presenting separate granules close to the cells suggesting some degranulation was also increased in asthma samples. In comparison to histopathological techniques based on brightfield microscopy, AFM showed considerably more details of these morphological changes, making the technique much more sensitive to detect eosinophil morphological changes that indicate functional alteration of this cell. AFM could be an important tool to evaluate diseases with alterations in eosinophil functions.
Identifiants
pubmed: 31632296
doi: 10.3389/fphys.2019.01261
pmc: PMC6781654
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1261Informations de copyright
Copyright © 2019 Eaton, Amaral, Couto, Oliveira, Vasconcelos, Borges, Kückelhaus, Leite and Muniz-Junqueira.
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